QTL mapping revealed TaVp-1A conferred pre-harvest sprouting resistance in wheat population Yanda 1817×Beinong 6

doi:10.1016/S2095-3119(16)61361-8

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (02): 435-444.DOI: 10.1016/S2095-3119(16)61361-8

收稿日期:2016-02-01 出版日期:2017-02-20 发布日期:2017-01-31

QTL mapping revealed TaVp-1A conferred pre-harvest sprouting resistance in wheat population Yanda 1817×Beinong 6

ZHOU Sheng-hui¹, FU Lin¹, WU Qiu-hong², CHEN Jiao-jiao¹, CHEN Yong-xing¹, XIE Jing-zhong¹, WANG Zhen-zhong³, WANG Guo-xin¹, ZHANG De-yun¹, LIANG Yong¹, ZHANG Yan¹, YOU Ming-shan¹, LIANG Rong-qi¹, HAN Jun⁴, LIU Zhi-yong^{1, 2}

¹State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China
²Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, P.R.China
³China Rural Technology Development Center, Beijing 100045, P.R.China
⁴College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, P.R.China

Received:2016-02-01 Online:2017-02-20 Published:2017-01-31
Contact: LIU Zhi-yong, Tel: +86-10-64806422, E-mail: zyliu@genetics.ac.cn
About author:ZHOU Sheng-hui, E-mail: zhoushenghui826@gmail.com
Supported by:
This work was financially supported by the National Natural Science Foundation of China (31271710, 31301312).

摘要/Abstract

Abstract: Pre-harvest sprouting (PHS) occurs frequently in most of the wheat cultivation area worldwide, which severely reduces yield and end-use quality, resulting in substantial economic loss. In this study, quantitative trait loci (QTL) for PHS resistance were mapped using an available high-density single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) genetic linkage map developed from a 269 recombinant inbred lines (RILs) population of Yanda 1817×Beinong 6. Using phenotypic data on two locations (Beijing and Shijiazhuang, China) in two years (2012 and 2013 harvesting seasons), five QTLs, designated as QPhs.cau-3A.1, QPhs.cau-3A.2, QPhs.cau-5B, QPhs.cau-4A, and QPhs.cau-6A, for PHS (GP) were detected by inclusive composite interval mapping (ICIM) (LOD≥2.5). Two major QTLs, QPhs.cau-3A.2 and QPhs.cau-5B, were mapped on 3AL and 5BS chromosome arms, explaining 6.29–21.65% and 4.36–5.94% of the phenotypic variance, respectively. Precise mapping and comparative genomic analysis revealed that the TaVp-1A flanking region on 3AL is responsible for QPhs.cau-3A.2. SNP markers flanking QPhs.cau-3A.2 genomic region were developed and could be used for introgression of PHS tolerance into high yielding wheat varieties through marker-assisted selection (MAS).

Key words: wheat, pre-harvest sprouting, quantitative trait loci, SNP, TaVp-1A

ZHOU Sheng-hui, FU Lin, WU Qiu-hong, CHEN Jiao-jiao, CHEN Yong-xing, XIE Jing-zhong, WANG Zhen-zhong, WANG Guo-xin, ZHANG De-yun, LIANG Yong, ZHANG Yan, OU Ming-shan, LIANG Rong-qi, HAN Jun, LIU Zhi-yong. QTL mapping revealed TaVp-1A conferred pre-harvest sprouting resistance in wheat population Yanda 1817×Beinong 6[J]. Journal of Integrative Agriculture, 2017, 16(02): 435-444.

参考文献

Allen G, Flores-Vergara M, Krasynanski S, Kumar S, Thompson W. 2006. A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide. Nature Protocols, 1, 2320–2325.
Ceccato D V, Bertero H D, Batlla D. 2011. Environmental control of dormancy in quinoa (Chenopodium quinoa) seeds: Two potential genetic resources for pre-harvest sprouting tolerance. Seed Science Research, 21, 133–141.
Chang C, Zhang H P, Feng J M, Yin B, Si H Q, Ma C X. 2010. Identifying alleles of Viviparous-1B associated with pre-harvest sprouting in micro-core collections of Chinese wheat germplasm. Molecular Breeding, 25, 481–490.
Chang C, Zhang H P, Zhao Q X, Feng J M, Si H Q, Lu J, Ma C X. 2011. Rich allelic variations of Viviparous-1A and their associations with seed dormancy/pre-harvest sprouting of common wheat. Euphytica, 179, 343–353.
Cowan A K. 2002. Pre-harvest sprouting: Safari in southern Africa. Euphytica, 126, 1–2.
Depauw R M, Mccaig T N. 1991. Components of variation, heritabilities and correlations for indexes of sprouting tolerance and seed dormancy in Triticum spp. Euphytica, 52, 221–229.
Flintham J, Adlam R, Bassoi M, Holdsworth M, Gale M. 2002. Mapping genes for resistance to sprouting damage in wheat. Euphytica, 126, 39–45.
Flintham J E. 2000. Different genetic components control coat-imposed and embryo-imposed dormancy in wheat. Seed Science Research, 10, 43–50.
Fofana B, Humphreys D G, Rasul G, Cloutier S, Brule-Babel A, Woods S, Lukow O M, Somers D J. 2009. Mapping quantitative trait loci controlling pre-harvest sprouting resistance in a red×white seeded spring wheat cross. Euphytica, 165, 509–521.
Gale M D. 1989. The genetics of preharvest sprouting in cereals, particularly in wheat. In: Derera N F, ed., Preharvest Field Sprouting in Cereals. CRC Press, USA. pp. 85–110.
Gale M D, Flintham J E, Devos K M. 2002. Cereal comparative genetics and preharvest sprouting. Euphytica, 126, 21–25.
Groos C, Gay G, Perretant M R, Gervais L, Bernard M, Dedryver F, Charmet D. 2002. Study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a whitexred grain bread-wheat cross. Theoretical and Applied Genetics, 104, 39–47.
Hagemann M G, Ciha A J. 1984. Evaluation of methods used in testing winter-wheat susceptibility to preharvest sprouting. Crop Science, 24, 249–254.
Holland J B, Nyquist W E, Cervantes-Martinez C T. 2003. Plant breeding reviews. In: Janick J, ed., Estimating and Interpreting Heritability for Plant Breeding: An Update. John Wiley & Sons. pp. 9–112.
Huang T, Qu B, Li H P, Zuo D Y, Zhao Z X, Liao Y C. 2012. A maize viviparous 1 gene increases seed dormancy and preharvest sprouting tolerance in transgenic wheat. Journal of Cereal Science, 55, 166–173.
IBI (The International Brachypodium Initiative). 2010. Genome sequencing and analysis of the model grass Brachypodium distachyon. Nature, 463, 763–768.
Incoln L, Ander E. 1993. Constructing Genetic Linkage Maps with MAPMAKER/EXP Version 3.0: A Tutorial and Reference Manual. Lander ES, Cambridge. pp. 1–9.
IRGSP (International Rice Genome Sequencing Project). 2005. The mapbased sequence of the rice genome. Nature, 436, 793–800.
King R W, Richards R A. 1984. Water-uptake in relation to pre-harvest sprouting damage in wheat-ear characteristics. Australian Journal of Agricultural Research, 35, 327–336.
Kulwal P L, Kumar N, Gaur A, Khurana P, Khurana J P, Tyagi A K, Balyan H S, Gupta P K. 2005. Mapping of a major QTL for pre-harvest sprouting tolerance on chromosome 3A in bread wheat. Theoretical and Applied Genetics, 111, 1052–1059.
Kulwal P L, Singh R, Balyan H S, Gupta P K. 2004. Genetic basis of pre-harvest sprouting tolerance using single-locus and two-locus QTL analyses in bread wheat. Functional & Integrative Genomics, 4, 94–101.
Kumar A, Kumar J, Singh R, Garg T, Chhuneja P, Balyan H S, Gupta P K. 2009. QTL analysis for grain colour and pre-harvest sprouting in bread wheat. Plant Science, 177, 114–122.
Li C D, Ni P X, Francki M, Hunter A, Zhang Y, Schibeci D, Li H, Tarr A, Wang J, Cakir M, Yu J, Bellgard M, Lance R, Appels R. 2004. Genes controlling seed dormancy and pre-harvest sprouting in a rice-wheat-barley comparison. Functional & Integrative Genomics, 4, 84–93.
Liu S B, Bai G H. 2010. Dissection and fine mapping of a major QTL for preharvest sprouting resistance in white wheat Rio Blanco. Theoretical and Applied Genetics, 121, 1395–1404.
Liu S B, Cai S B, Graybosch R, Chen C X, Bai G H. 2008. Quantitative trait loci for resistance to pre-harvest sprouting in US hard white winter wheat Rio Blanco. Theoretical and Applied Genetics, 117, 691–699.
Liu S B, Sehgal S K, Li J R, Lin M, Trick H N, Yu J M, Gill B S, Bai G H. 2013. Cloning and characterization of a critical regulator for preharvest dprouting in wheat. Genetics, 195, 263.
McCarty D R, Hattori T, Carson C B, Vasil V, Lazar M, Vasil I K. 1991. The Viviparous-1 developmental gene of maize encodes a novel transcriptional activator. Cell, 66, 895–905.
McIntosh R A, Yamazaki Y, Dubcovsky J, rogers J, Morris C, Appelsr, Xia X C. 2013. Catalogue of gene symbols for wheat. In: 12 th International Wheat Genetics Symposium, Yokohama, Japan. Springer, Germany.
McKibbin R S, Wilkinson M D, Bailey P C, Flintham J E, Andrew L M, Lazzeri P A, Gale M D, Lenton J R, Holdsworth M J. 2002. Transcripts of Vp-1 homeologues are misspliced in modern wheat and ancestral species. Proceedings of the National Academy of Sciences of the United States of America, 99, 10203–10208.
Miao X L, Zhang Y J, Xia X C, He Z H, Zhang Y, Yan J, Chen X M. 2013. Mapping quantitative trait loci for pre-harvest sprouting resistance in white-grained winter wheat line CA 0431. Crop & Pasture Science, 64, 573–579.
Mohan A, Kulwal P, Singh R, Kumar V, Mir R R, Kumar J, Prasad M, Balyan H S, Gupta P K. 2009. Genome-wide QTL analysis for pre-harvest sprouting tolerance in bread wheat. Euphytica, 168, 319–329.
Mori M, Uchino N, Chono M, Kato K, Miura H. 2005. Mapping QTLs for grain dormancy on wheat chromosome 3A and the group 4 chromosomes, and their combined effect. Theoretical and Applied Genetics, 110, 1315–1323.
Munkvold J D, Tanaka J, Benscher D, Sorrells M E. 2009. Mapping quantitative trait loci for preharvest sprouting resistance in white wheat. Theoretical and Applied Genetics, 119, 1223–1235.
Nakamura S, Abe F, Kawahigashi H, Nakazono K, Tagiri A, Matsumoto T, Utsugi S, Ogawa T, Handa H, Ishida H, Mori M, Kawaura K, Ogihara Y, Miura H. 2011. A Wheat homolog of MOTHER OF FT AND TFL1 Acts in the regulation of germination. The Plant Cell, 23, 3215–3229.
Nakamura S, Toyama T. 2001. Isolation of a VP1 homologue from wheat and analysis of its expression in embryos of dormant and non-dormant cultivars. Journal of Experimental Botany, 52, 1952–1952.
Osa M, Kato K, Mori M, Shindo C, Torada A, Miura H. 2003. Mapping QTLs for seed dormancy and the Vp1 homologue on chromosome 3A in wheat. Theoretical and Applied Genetics, 106, 1491–1496.
Ouyang S H, Zhang D, Han J, Zhao X J, Cui Y, Song W, Huo N X, Liang Y, Xie J Z, Wang Z Z, Wu Q H, Chen Y X, Lu P, Zhang D Y, Wang L L, Sun H, Yang T M, Keeble-Gagnere G, Appels R, Dolezel J, et al. 2014. Fine physical and genetic mapping of powdery mildew resistance gene MlIW172 originating from wild emmer (Triticum dicoccoides). PLOS ONE, 9, e100160.
Paterson A H, Bowers J E, Bruggmann R, Dubchak I, Grimwood J, Gundlach H, Haberer G, Hellsten U, Mitros T, Poliakov A, Schmutz J, Spannagl M, Tang H B, Wang X Y, Wicker T, Bharti A K, Chapman J, Feltus F A, Gowik U, Grigoriev I V, et al. 2009. The Sorghum bicolor genome and the diversification of grasses. Nature, 457, 551–556.
Singh R, Matus-Cadiz M, Baga M, Hucl P, Chibbar R N. 2010. Identification of genomic regions associated with seed dormancy in white-grained wheat. Euphytica, 174, 391–408.
Sun Y W, Jones H D, Yang Y, Dreisigacker S, Li S M, Chen X M, Shewry P R, Xia L Q. 2012. Haplotype analysis of Viviparous-1 gene in CIMMYT elite bread wheat germplasm. Euphytica, 186, 25–43.
Wahl T I, Orourke A D. 1993. The Economics of sprout damage in wheat. In: Pre-Harvest Sprouting in Cereals 1992. AACC International Press, USA. pp. 10–17.
Wang Z Z, Cui Y, Chen Y X, Zhang D Y, Liang Y, Zhang D, Wu Q H, Xie J Z, Ouyang S H, Li D L, Huang Y L, Lu P, Wang G X, Yu M H, Zhou S H, Sun Q X, Liu Z Y. 2014. Comparative genetic mapping and genomic region collinearity analysis of the powdery mildew resistance gene Pm41. Theoretical and Applied Genetics, 127, 1741–1751.
Wu H B, Qin J X, Han J, Zhao X J, Ouyang S H, Liang Y, Zhang D, Wang Z Z, Wu Q H, Xie J Z, Cui Y, Peng H R, Sun Q X, Liu Z Y. 2013. Comparative high-resolution mapping of the wax inhibitors Iw1 and Iw2 in hexaploid wheat. PLOS ONE, 8, e84691.
Wu Q H, Chen Y X, Zhou S H, Fu L, Chen J J, Xiao Y, Zhang D, Ouyang S H, Zhao X J, Cui Y, Zhang D Y, Liang Y, Wang Z Z, Xie J Z, Qin J X, Wang G X, Li D L, Huang Y L, Yu M H, Lu P, et al. 2015. High-density genetic linkage map construction and QTL mapping of grain shape and size in the wheat population Yanda1817×Beinong6. PLOS ONE, 10, e0118144.
Xiao S H, Zhang H P, You G X, Zhang X Y, Yan C S, Chen X. 2012. Integration of marker-assisted selection for resistance to pre-harvest sprouting with selection for grain-filling rate in breeding of white-kernelled wheat for the Chinese environment. Euphytica, 188, 85–88.
Yang Y, Ma Y Z, Xu Z S, Chen X M, He Z H, Yu Z, Wilkinson M, Jones H D, Shewry P R, Xia L Q. 2007. Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance. Journal of Experimental Botany, 58, 2863–2871.
Yang Y, Zhang C L, Liu S X, Sun Y Q, Meng J Y, Xia L Q. 2014. Characterization of the rich haplotypes of Viviparous-1A in Chinese wheats and development of a novel sequence-tagged site marker for pre-harvest sprouting resistance. Molecular Breeding, 33, 75–88.
You F M, Huo N, Gu Y Q, Luo M C, Ma Y, Hane D, Lazo G R, Dvorak J, Anderson O D. 2008. BatchPrimer3: A high throughput web application for PCR and sequencing primer design. BMC Bioinformatics, 9, 253.
Zanetti S, Winzeler M, Keller M, Keller B, Messmer M. 2000. Genetic analysis of pre-harvest sprouting resistance in a wheat×spelt cross. Crop Science, 40, 1406–1417.

QTL mapping revealed TaVp-1A conferred pre-harvest sprouting resistance in wheat population Yanda 1817×Beinong 6

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics