Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (8): 1798-1807.DOI: 10.1016/S2095-3119(16)61344-8

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  • 收稿日期:2015-10-09 出版日期:2016-08-01 发布日期:2016-08-01

Imazamox microbial degradation by common clinical bacteria: Acinetobacter baumannii IB5 isolated from black soil in China shows high potency

LIU Chun-guang1, 2, 3, YANG Xin1, LAI Yang1, LU Hong-gang1, ZENG Wei-min1, 2, GENG Gui3, 4, YANG Feng-shan1, 2   

  1. 1 Key Laboratory of Microbiology, Life Science College, Heilongjiang University, Harbin 150080, P.R.China
    2 Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Harbin 150501, P.R.China
    3 Sugar Beet Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150080, P.R.China
    4 Crop Research Institute, Heilongjiang University, Harbin 150080, P.R.China
  • Received:2015-10-09 Online:2016-08-01 Published:2016-08-01
  • Contact: YANG Feng-shan, Tel/Fax: +86-451-86608001, E-mail: yangfengshan@hlju.edu.cn
  • About author:LIU Chun-guang, E-mail: 2005013@hlju.edu.cn;
  • Supported by:

    This work was supported by the Public Welfare Industry Re­search Project, Ministry of Agriculture of China (201203098), and the Harbin Applied Technology Research and Development Project, China (Youth Reserve Talents Class A: 2015RAQYJ009).

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Abstract:   Herbicidal residues of imazamox are hazardous to some sensitive rotational aftercrops. The aim of the study was to isolate and identify a microbial strain capable of degrading imazamox. The strain IB5, capable of efficiently degrading imazamox, was isolated from an imazamox-contaminated soybean field in Heilongjiang Province, China. It was found to degrade 98.61% of 400 mg L–1 imazamox within 48 h by high-performance liquid chromatograph. Through morphological, physiological and biochemical characterization, and the 16S rDNA sequencing, the strain was identified as Acinetobacter baumannii. An optimal degradation condition was obtained and verified: 400 mg L–1 imazamox, 0.1% (volume ratio) initial inoculum, 37°C and pH 7.0. Four main products were captured in the liquid chromatograms and mass spectra, and a pathway for imazamox degradation by IB5 was proposed. This work provides a new suitable candidate for imazamox biodegradation and theoretical evidence for imazamox residue bioremediation. A. baumannii is a common clinical bacteria, but its imazamox-degrading feature has not been reported previously.

Key words: imazamox ,  biodegradation ,  Acinetobacter baumannii ,  metabolites ,  herbicide