A novel strategy to enhance resistance to Cucumber mosaic virus in tomato by grafting to transgenic rootstocks

doi:10.1016/S2095-3119(16)61330-8

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (9): 2040-2048.DOI: 10.1016/S2095-3119(16)61330-8

收稿日期:2015-11-12 出版日期:2016-09-02 发布日期:2016-09-02

A novel strategy to enhance resistance to Cucumber mosaic virus in tomato by grafting to transgenic rootstocks

BAI Miao^1*, CHEN Wen-ting^1*, XIE Bing-yan², YANG Guo-shun¹

¹ College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, P.R.China
² Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2015-11-12 Online:2016-09-02 Published:2016-09-02
Contact: YANG Guo-shun, Tel: +86-731-84618767, Fax: +86-731-84618171, E-mail: guoshunyang@foxmail.com
About author:BAI Miao, Mobile: +86-18374879885, E-mail: baimiao1984@qq.com;
Supported by:
This work was supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China (20134320120013) and the Natural Science Foundation of Hunan Province of China (14JJ3095).

摘要/Abstract

Abstract: Cucumber mosaic virus (CMV) can infect a wide range of host species. For the lacking of CMV resistant varieties of tomato, RNA interference (RNAi) can be used as a fast and effective method for the generation of transgenic resistant varieties. In this current study, five intron-spliced hairpin RNA (ihpRNA) plant expression vectors aimed at five genes of CMV have been constructed. Transgenic tomatoes were obtained by Agrobacterium tumefaciens-mediated transformation with expression vectors. Highly resistant generations of transgenic plants were employed as rootstocks and grafted onto non-transgenic tomatoes that resulted in the successful transfer of resistance to the scions. Using a novel method of plant cuttings for rootstock propagation, we obtained large quantities of disease-resistant material. Further, this method produces scions that can remain undetectable for transgenic resistance marker genes that may provide novel approaches to evade collective concerns about genetically-modified organism (GMO) biosafety.

Key words: Cucumber mosaic virus (CMV) , graft , RNA silencing , Solanum lycopersicum , virus resistance

BAI Miao, CHEN Wen-ting, XIE Bing-yan, YANG Guo-shun. [J]. Journal of Integrative Agriculture, 2016, 15(9): 2040-2048.

BAI Miao, CHEN Wen-ting, XIE Bing-yan, YANG Guo-shun. A novel strategy to enhance resistance to Cucumber mosaic virus in tomato by grafting to transgenic rootstocks[J]. Journal of Integrative Agriculture, 2016, 15(9): 2040-2048.

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A novel strategy to enhance resistance to Cucumber mosaic virus in tomato by grafting to transgenic rootstocks

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