The sigma 54 genes rpoN1 and rpoN2 of Xanthomonas citri subsp. citri play different roles in virulence, nutrient utilization and cell motility

doi:10.1016/S2095-3119(15)61317-X

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (9): 2032-2039.DOI: 10.1016/S2095-3119(15)61317-X

收稿日期:2015-11-27 出版日期:2016-09-02 发布日期:2016-09-02

The sigma 54 genes rpoN1 and rpoN2 of Xanthomonas citri subsp. citri play different roles in virulence, nutrient utilization and cell motility

Gibson Kamau Gicharu¹, SUN Dong-ling¹, HU Xun¹, FAN Xiao-jing¹, ZHUO Tao¹, WU Chuan-wan², ZOU Hua-song¹

¹ Fujian Province Key Laboratory of Plant Virology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China
² Huai’an Institute of Agricultural Sciences, Huai’an 223001, P.R.China

Received:2015-11-27 Online:2016-09-02 Published:2016-09-02
Contact: ZOU Hua-song, Tel: +86-591-8378469, E-mail: hszou@fafu.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (31171832) and the Jiangsu Agriculture Science and Technology Innovation Fund, China (CX(11)4056).

摘要/Abstract

Abstract: The sigma factor 54 (σ⁵⁴) controls the expression of many genes in response to nutritional and environmental conditions. There are two σ⁵⁴ genes, rpoN1 (XAC1969) and rpoN2 (XAC2972), in Xanthomonas citri subsp. citri. To investigate their functions, the deletion mutants ΔrpoN1, ΔrpoN2 and ΔrpoN1N2 were constructed in this study. All the mutants delayed canker development in low concentration inoculation in citrus plants. The bacterial growth of mutants was retarded in the medium supplemented with nitrogen and carbon resources. Under either condition, the influence degree caused by deletion of rpoN2 was larger than the deletion of rpoN1. Remarkably, the mutant ΔrpoN1 showed a reduction in cell motility, while the mutant ΔrpoN2 increased cell motility. Our data suggested that the rpoN1 and rpoN2 play diverse roles in X. citri subsp. citri.

Key words: Xanthomonas citri subsp. citri , rpoN , cell motility , citrus canker , full virulence

Gibson Kamau Gicharu, SUN Dong-ling, HU Xun, FAN Xiao-jing, ZHUO Tao, WU Chuan-wan, ZOU Hua-song. [J]. Journal of Integrative Agriculture, 2016, 15(9): 2032-2039.

Gibson Kamau Gicharu, SUN Dong-ling, HU Xun, FAN Xiao-jing, ZHUO Tao, WU Chuan-wan, ZOU Hua-song. The sigma 54 genes rpoN1 and rpoN2 of Xanthomonas citri subsp. citri play different roles in virulence, nutrient utilization and cell motility[J]. Journal of Integrative Agriculture, 2016, 15(9): 2032-2039.

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The sigma 54 genes rpoN1 and rpoN2 of Xanthomonas citri subsp. citri play different roles in virulence, nutrient utilization and cell motility

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