Phenotypic characterization and fine mapping of mps1, a premature leaf senescence mutant in rice (Oryza sativa L.)

doi:10.1016/S2095-3119(15)61279-5

摘要/Abstract

Abstract: Leaves play a key role in photosynthesis in rice plants. The premature senescence of such plants directly reduces the accumulation of photosynthetic products and also affects yield and grain quality significantly and negatively. A novel premature senescence mutant, mps1 (mid-late stage premature senescence 1), was identified from a mutant library consisting of ethyl methane sulfonate (EMS) induced descendants of Jinhui 10, an elite indica restorer line of rice. The mutant allele, mps1, caused no phenotypic differences from the wild type (WT), Jinhui 10, but drove the leaves to turn yellow when mutant plants grew to the tillering stage, and accelerated leaf senescence from the filling stage to final maturation. We characterized the agronomic traits, content of photosynthetic pigments and photosynthetic efficiency of mps1 and WT, and fine-mapped MPS1. The results showed that the MPS1-drove premature phenotype appeared initially on the leaf tips at the late tillering stage and extended to the middle of leaves during the maturing stage. Compared to the WT, significant differences were observed among traits of the number of grains per panicle (–31.7%) and effective number of grains per panicle (–38.5%) of mps1 individuals. Chlorophyll contents among the first leaf from the top (Top 1st), the second leaf from the top (Top 2nd) and the third leaf from the top (Top 3rd) of mps1 were significantly lower than those of WT (P<0.05), and the levels of photosynthetic efficiency from Top 1st to the forth leaf from the top (Top 4th) of mps1 were significantly lower than those of WT (P<0.01). Results from the genetic analysis indicated that the premature senescence of mps1 is controlled by a recessive nuclear gene, and this locus, MPS1 is located in a 37.4-kb physical interval between the markers Indel145 and Indel149 on chromosome 6. Genomic annotation suggested eight open reading frames (ORFs) within this physical region. All of these results will provide informative references for the further researches involving functional analyses and molecular mechanism exploring of MPS1 in rice.

Key words: rice (Oryza sativa L.) , premature senescence , fine mapping , mps1

LIU Zhong-xian, CUI Yu, WANG Zhong-wei, XIE Yuan-hua, SANG Xian-chun, YANG Zheng-lin, ZHANG Chang-wei, ZHAO Fang-ming, HE Guang-hua, LING Ying-hua. [J]. Journal of Integrative Agriculture, 2016, 15(9): 1944-1954.

LIU Zhong-xian, CUI Yu, WANG Zhong-wei, XIE Yuan-hua, SANG Xian-chun, YANG Zheng-lin, ZHANG Chang-wei, ZHAO Fang-ming, HE Guang-hua, LING Ying-hua. Phenotypic characterization and fine mapping of mps1, a premature leaf senescence mutant in rice (Oryza sativa L.)[J]. Journal of Integrative Agriculture, 2016, 15(9): 1944-1954.

参考文献

Ansari M I, Chen S C G. 2009. Biochemical characterization of gamma-aminobutyric acid (GABA): Pyruvate transaminase during rice leaf senescence. International Journal of Integrative Biology, 6, 27–32.

Ansari M I, Chen S C G. 2011. Leaf senescence - An overview. International Journal of Recent Trends in Science and Technology, 1, 110–114.

Ansari M I, Lee R H, Chen S C G. 2005. A novel senescence-associated gene encoding γ-aminobutyric acid (GABA): Pyruvate transaminase is upregulated during rice leaf senescence. Physiologia Plantarum, 123, 1–8.

Chauhan J S, Mishra N K, Raghava G P S. 2009. Identification of ATP binding residues of a protein from its primary sequence. BMC Bioinformatics, 10, 434–452.

Chen C, Ke J, Zhou X E, Yi W, Brunzelle J S, Li J, Yong E L, Xu H E, Melcher K. 2013. Structural basis for molecular recognition of folic acid folate receptors. Nature, 500, 486–490.

Dai G M, Zhu X Y, Ling Y H, Zhao F M, Yang Z L, He G H. 2013. Genetic analysis and fine mapping of a lesion mimic mutant spl31 in rice. Acta Agronomica Sinica, 39, 1223–1230. (in Chinese)

Du Q, Fang L K, Sang X C, Ling Y H, Li Y F, Yang Z L, He G H, Zhao F M. 2012. Analysis of phenotype and physiology of leaf apex dead mutant (lad) in rice and mapping of mutant gene. Acta Agronomica Sinica, 38, 168–173. (in Chinese)

Fang L K, Li Y F, Gong X P, Sang X C, Ling Y H, Wang X W, Cong Y F, He G H. 2010. Genetic analysis and gene mapping of dominant presenescing leaf gene PSL3 in rice (Oryza sativa L.). Chinese Science Bulletin, 55, 2517–2521.

Gan S, Amasino R M. 1997. Making sense of senescence. Plant Physiology, 113, 313–319.

Gepstein S, Sabehi G, Carp M J, Hajouj T, Mesher M F O, Yariv I, Dor C, Bassani M. 2003. Large-scale identification of leaf senescence-associated genes. The Plant Journal, 36, 629–642.

Guo S, Zhang T, Xing Y, Zhu X, Sang X, Ling Y, Wang N, He G. 2014. Identification and gene mapping of an early senescence leaf 4 mutant of rice. Crop Science, 54, 2713–2723.

Huang Q N, Shi Y F, Zhang X B, Song L X, Feng B H, Wang H M, Xu X, Li X H, Guo D, Wu J L. 2015. Single base substitution in OsCDC48 is responsible for premature senescence and death phenotype in rice. Journal of Integrative Plant Biology, 58, 12–28.

Jaiswal N, Saraswat S, Ratnam M, Isailovic D. 2012. Analysis of folate binding protein N-linked glycans by mass spectrometry. Journal of Proteome Research, 11, 1551–1560.

Jiang H, Chen Y, Li M, Xu X, Wu G. 2011. Overexpression of SGR results in oxidative stress and lesion-mimic cell death in rice seedlings. Journal of Integrative Plant Biology, 53, 375–387.

Jiao B B, Wang J J, Zhu X D, Zeng L J, Li Q, He Z H. 2012. A novel protein RLS1 with NB-ARM domains is involved in chloroplast degradation during leaf senescence in rice. Molecular Plant, 5, 205–217.

Kobayashi N I, Saito T, Iwata N, Ohmae Y, Iwata R, Tanoi K, Makanishi T M. 2012. Leaf senescence in rice due to magnesium deficiency mediated defect in transpiration rate before sugar accumulation and chlorosis. Physiologia Plantarum, 148, 490–501.

Lee R H, Chen S C G. 2002. Programmed cell death during rice leaf senescence is nonapoptotic. New Phytologist, 155, 25–32.

Li F Z, Hu G C, Fu Y P, Si H M, Bai X M, Sun Z X. 2005. Genetic analysis and high-resolution mapping of a premature senescence gene Pse(t) in rice (Oryza sativa L.). Genome, 48, 738–746.

Li W, Wu J, Weng S, Zhang Y, Zhang D, Shi C. 2010. Identification and characterization of dwarf 62, a loss-of-function mutation in DLT/OsGRAS-32 affecting gibberellin metabolism in rice. Planta, 232, 1383–1396.

Li Y, Wang J K, Qiu L J, Ma Y Z, Li X H, Wan J M. 2010. Crop molecular breeding in China: Current status and perspectives. Acta Agronomica Sinica, 36, 1425–1430. (in Chinese)

Li Z, Zhao Y, Liu X, Peng J, Guo H, Luo J. 2013. LSD 2.0: An update of the leaf senescence database. Nucleic Acids Research, 42, D1200–D1205.

Liang C, Wang Y, Zhu Y, Tang J, Hu B, Liu L, Ou S, Wu H, Sun X, Chu J, Chu C. 2014. OsNAP connects abscisic acid and leaf senescence by fine-tuning abscisic acid biosynthesis and directly targeting senescence-associated genes in rice. Proceedings of the National Academy of Sciences of the United States of America, 111, 10013–10018.

Lim P O, Kim H J, Nam H G. 2007. Leaf senescence. Annual Review of Plant Biology, 58, 115–136.

Lin A H, Wang Y Q, Tang J Y, Xue P, Li C L, Liu L C, Hu B, Yang F Q, Loake G J, Chu C C. 2012. Nitric oxide and protein S-nitrosylation are integral to hydrogen peroxide induced leaf cell death in rice. Plant Physiology, 158, 1451–1464.

Liu T, Zeng J, Xia K, Fan T, Li Y, Wang Y, Xu X, Zhang M. 2012. Evolutionary expansion and functional diversification of oligopeptide transporter gene family in rice. Rice, 5, 12–26.

Liu X, Li Z, Jiang Z, Zhao Y, Peng J, Jin J, Guo H, Luo J. 2011. LSD: A leaf senescence database. Nucleic Acids Research, 39, D1103–D1107.

Miao R L, Jiang Y D, Liao H X, Xu F F, He G H, Yang Z L, Zhao F M, Sang X C. 2013. Identification and gene mapping of rice early senescence leaf (esl3) mutant. Acta Agronomica Sinica, 39, 862–867. (in Chinese)

Morita R, Sato Y, Masuda Y, Nishimura M, Kusaba M. 2009. Defect in non-yellow coloring 3, an α/β hydrolase-fold family protein, causes a stay-green phenotype during leaf senescence in rice. The Plant Journal, 59, 940–952.

Nooden L D, Guiamet J J, John I. 1997. Senescence mechanisms. Physiological Planta, 101, 746–753.

Park S Y, Yu J W, Park J S, Li J J, Yoo S C, Lee N Y, Lee S K, Jeong S W, Seo H S, Koh H J, Jeon J S, Park Y, Paek N C. 2007. The senescence-induced stay green protein regulates chlorophyll degradation. The Plant Cell, 19, 1649–1664.

Qiao Y, Jiang W, Lee J, Park B, Choi M S, Piao R, Woo M O, Roh J H, Han L Z, Paek N C, Seo H S, Koh H J. 2010. SPL28 encodes a clathrin-associated adaptor protein complex 1, medium subunit mu1 (AP1M1) and is responsible for spotted leaf and early senescence in rice (Oryza sativa). New Phytologist, 185, 258–274.

Rong H, Tang Y, Zhang H, Wu P, Chen Y, Li M, Wu G, Jiang H. 2013. The stay-green rice like (SGRL) gene regulates chlorophyll degradation in rice. Journal of Plant Physiology, 170, 1367–1373.

Saghai-Maroof M A, Soliman K M, Jorgenson R, Allward R W. 1984. Ribosomal DNA spacer length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proceedings of National Academy of Sciences of the United States of America, 81, 8014–8018.

Sang X C, Xu F F, Zhu X Y, Xing Y D, He P L, Zhang C W, Yang Z L, He G H. 2014. Identification and gene fine mapping of early senescence leaf mutant esl5 in Oryza sativa. Acta Agronomica Sinica, 40, 1182–1189. (in Chinese)

Sato T, Miyanoiri Y, Takeda M, Naoe Y, Mitani R, Hirano K, Takehara S, Kainosho M, Matsuoka M, Ueguchi-Tanaka M, Kato H. 2014. Expression and purification of a GRAS domain of SLR1, the rice DELLA protein. Protein Expression and Purification, 95, 248–258.

Sato Y, Morita R, Katsuma S, Nishimura M, Tanaka A, Kusaba M. 2009. Two short-chain dehydrogenase/reductase, NON-YELLOW COLORING 1 and NYC1-LIKE, are required for chlorophyll b and light-harvesting complex II degradation during senescence in rice. The Plant Journal, 57, 120–131.

Schippers J H M. 2015. Transcriptional networks in leaf senescence. Current Opinion in Plant Biology, 27, 77–83.

Shi B, Ni L, Zhang A, Cao J, Zhang H, Qin T, Tan M, Zhang J, Jiang M. 2012. OsDMI3 is a novel component of abscisic acid signaling in the induction of antioxidant defense in leaves of rice. Molecular Plant, 5, 1359–1374.

Shi B, Ni L, Zhang A, Tan M, Jiang M. 2014. OsDMI3 is a novel component of abscisic acid signaling in the induction of antioxidant defense in leaves of rice. Plant, Cell and Environment, 37, 341–352.

Sudo E, Itouga M, Yoshida-Hatanaka K, Ono Y, Sakakibara H. 2008. Gene expression and sensitivity in response to copper stress in rice leaves. Journal of Experimental Botany, 59, 3465–3474.

Tester M, Langridge P. 2010. Breeding technologies to increase crop production in a changing world. Science, 327, 818–822.

Van Wilder, De Brouwer V, Loizeau K, Gambonner B, Albrieux C, Van Der Straeten D, Lambert W E, Douce R, Block M A, Rebeille F, Ravanel S. 2009. C1 metabolism and chlorophyll synthesis: The Mg-protoporphyrin IX methyltransferase activity is dependent on the folate status. New Phytologist, 182, 137–145.

Wang J, Wu S J, Zhou Y, Zhou L H, Xu J F, Hu J, Fang Y X, Gu M H, Liang G H. 2006. Genetic analysis and molecular mapping of a presenescing leaf gene psl1 in rice (Oryza sativa L.). Chinese Science Bulletin, 51, 2986–2992.

Webb M E, Smith A G. 2009. Cholophyll and folate: Intimate link revealed by drug treatment. New Phytologist, 182, 3–5.

Wei G, Tao Y, Liu G, Chen C, Luo R, Xia H, Gan Q, Zeng H, Lu Z, Han Y, Li X, Song G, Zhai H, Peng Y, Li D, Xu H, Wei X, Cao M, Deng H, Xin Y, et al. 2009. A transcriptomic analysis of superhybrid rice LYP9 and its panrets. Proceedings of the National Academy of Sciences of the United States of America, 106, 7695–7701.

Wu X Y, Kuai B K, Jia J Z, Jing H C. 2012. Regulation of leaf senescence and crop genetic improvement. Journal of Integrative Plant Biology, 54, 936–952.

Xu F F, Sang X C, Ren D Y, Tang Y Q, Hu H W, Yang Z L, Zhao F M, He G H. 2012. Genetic analysis and gene mapping of early senescence leaf mutant esl2 in rice. Acta Agronomica Sinica, 38, 1347–1353. (in Chinese)

Yamatani H, Sato Y, Masuda Y, Kato Y, Moria R, Fukunaga K, Nagamura Y, Nishimura M, Sakamoto W, Tanaka A, Kusaba M. 2013. NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll-protein complexes during leaf senescence. The Plant Journal, 74, 652–662.

Yan W Y, Ye S H, Jin Q S, Zeng L J, Peng Y, Yan D W, Yang W B, Yang D L, He Z H, Dong Y J, Zhang X M. 2010. Characterization and mapping of a novel mutant sms1 (senescence and male sterility 1) in rice. Journal of Genetics and Genomics, 37, 47–55.

Yang Y L, Rao Y C, Liu H J, Fang Y X, Dong G J, Huang L C, Leng Y J, Guo L B, Zhang G H, Hu J, Gao Z Y, Qian Q, Zeng D L. 2011. Characterization and fine mapping of an early senescence mutant (es-t) in Oryza sativa L. Chinese Science Bulletin, 56, 2437–2443.

Yoo S C, Cho S H, Sugimoto H, Li J, Jusumi K, Koh H J, Iba K, Paek N C. 2009. Rice virescent3 and stripe1 encoding the large and small subunits of ribonucleotide reductase are required for chloroplast biogenesis during early leaf development. Plant Physiology, 150, 388–401.

Yoshida S. 2003. Molecular regulation of leaf senescence. Current Opinion in Plant Biology, 6, 79–84.

Zhang T, Sun Y Y, Zheng J M, Cheng Z J, Jiang K F, Yang L, Cao Y J, You S M, Wan J M, Zheng J K. 2014. Genetic analysis and fine mapping of a premature leaf senescence mutant in rice (Oryza sativa L.). Acta Agronomica Sinica, 40, 2070–2080. (in Chinese)

Zhao C C, Huang F D, Gong P, Yang X, Cheng F M, Pan G. 2014. Physiological characteristics and gene mapping of a leaf early-senescence mutant osled in rice. Acta Agronomica Sinica, 40, 1946–1955. (in Chinese)

Zhou Y, Liu L, Huang W, Yuan M, Zhou F, Li X, Lin Y. 2013. Overexpression of OsSWEET5 in rice causes growth retardation and precocious senescence. PLOS ONE, 9, e94210.

Zhu L, Liu W Z, Wu C, Luan W J, Fu Y P, Hu G C, Si H M, Sun Z X. 2007. Identification and fine mapping of a gene related to pale green leaf phenotype near centromere region in rice (Oryza sativa L.). Rice Science, 14, 172–180.