Identification of a novel gain-of-function mutant allele, slr1-d5, of rice DELLA protein

doi:10.1016/S2095-3119(15)61208-4

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (7): 1441-1448.DOI: 10.1016/S2095-3119(15)61208-4

Identification of a novel gain-of-function mutant allele, slr1-d5, of rice DELLA protein

收稿日期:2015-06-09 出版日期:2016-07-06 发布日期:2016-07-06

Identification of a novel gain-of-function mutant allele, slr1-d5, of rice DELLA protein

ZHANG Yun-hui^1*, BIAN Xiao-feng^1*, ZHANG Suo-bing¹, LING Jing¹, WANG Ying-jie¹, WEI Xiao-ying², FANG Xian-wen¹

¹ The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm/Jiangsu Provincial Key Laboratory of Agrobiology/Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, P.R.China
² Shandong Business Institute, Yantai 264670, P.R.China

Received:2015-06-09 Online:2016-07-06 Published:2016-07-06
Contact: FANG Xian-wen, Tel: +86-25-84390321, E-mail: xianwen_fang@hotmail.com
About author:HANG Yun-hui, E-mail: zyhrice@163.com; BIAN Xiao-feng, E-mail: bianxiaofeng2@163.com
Supported by:
This work was supported by the National Natural Science Foundation of China (31401036), the Jiangsu Independent Innovation Project (CX(14)5005), the Natural Science Foundation of Jiangsu Province, China (BK20130706), and the Basal Research Fund of Jiangsu Acadamy of Agricultural Sciences, China (ZX(15)4015).

摘要/Abstract

Abstract: Controlling the height of crops plays a crucial role for their yields. The large scale utilization of semi-dwarf varieties has greatly improved crop yield, providing an effective support for world food security. In rice, a main food for over half of the world’s population, a number of dwarf loci have been identified. However, most of them are recessive, such as the ‘green revolution’ gene sd1. To gain more beneficial loci for rice breeding programs, exploring new mutations is needed, especially the dominant loci which can be used broadly for hybrid breeding. Here, we isolated a novel dominant dwarf rice mutant, slr1-d5. All of the internodes of slr1-d5 are reduced. We find that the responsiveness of slr1-d5 to gibberellin (GA), GA₃, was significantly reduced. Map-based cloning revealed that the dominant dwarfism of slr1-d5 was caused by an amino acid substitution in the N-terminal TVHYNP domain of rice DELLA protein, SLR1, where the conserved amino acid Pro (P) was substituted to His (H). Our findings not only further prove the pivotal role of TVHYNP motif in regulating SLR1 stability, but also provide a new dwarf source for improvement of rice germplasms.

Key words: rice , dominant dwarf , DELLA protein , gibberellin

ZHANG Yun-hui, BIAN Xiao-feng, ZHANG Suo-bing, LING Jing, WANG Ying-jie, WEI Xiao-ying, FANG Xian-wen. Identification of a novel gain-of-function mutant allele, slr1-d5, of rice DELLA protein[J]. Journal of Integrative Agriculture, 2016, 15(7): 1441-1448.

参考文献

Asano K, Hirano K, Ueguchi-Tanaka M, Angeles-Shim R B, Komura T, Satoh H, Kitano H, Matsuoka M, Ashikari M. 2009. Isolation and characterization of dominant dwarf mutants, Slr1-d, in rice. Molecular Genetics and Genomics, 281, 223–231.

Aasno K, Takashi T, Miura K, Qian Q, Kitano H, Matsuoka M, Ashikari M. 2007. Genetic and molecular analysis of utility of sd1 alleles in rice breeding. Breeding Science, 57, 53–58.

Chen X, Temnykh S, Xu Y, Cho Y G, McCouch S R. 1997. Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.). Theoretical and Applied Genetics, 95, 553–567.

Dellaporta S L, Wood J, Hicks J B. 1983. A plant DNA mini preparation: Version II. Plant Molecular Biology Reporter, 1, 19–21.

Dill A, Jung H S, Sun T P. 2001. The DELLA motif is essential for gibberellin-induced degradation of RGA. Proceedings of the National Academy of Sciences of the United States of America, 98, 14162–14167.

Fleet C M, Sun T P. 2005. A DELLAcate balance: The role of gibberellin in plant morphogenesis. Current Opinion in Plant Biology, 8, 77–85.

Gomi K, Sasaki A, Itoh H, Ueguchi-Tanaka M, Ashikari M, Kitano H, Matsuoka M. 2004. GID2, an F-box subunit of the SCF E3 complex, specifically interacts with phosphorylated SLR1 protein and regulates the gibberellin-dependent degradation of SLR1 in rice. The Plant Journal, 37, 626–634.

Griffiths J, Murase K, Rieu I, Zentella R, Zhang Z L, Powers S J, Gong F, Phillips A L, Hedden P, Sun T P, Thomas S G. 2006. Genetic characterization and functional analysis of the GID1 gibberellin receptors in Arabidopsis. The Plant Cell, 18, 3399–3414.

Hargrove T R, Cabanilla V L. 1979. The impact of semi-dwarf varieties on Asian rice-breeding programs. Bioscience, 29, 731–735.

Hedden P. 2003. The genes of the green revolution. Trends in Genetics, 19, 5–9.

Hedden P, Phillips A L. 2000. Gibberellin metabolism: New insights revealed by the genes. Trends in Plant Science, 5, 523–530.

Hirano K, Asano K, Tsuji H, Kawamura M, Mori H, Kitano H, Ueguchi-Tanaka M, Matsuoka M. 2010. Characterization of the molecular mechanism underlying gibberellin perception complex formation in rice. The Plant Cell, 22, 2680–2696.

Ikeda A, Ueguchi-Tanaka M, Sonoda Y, Kitano H, Koshioka M, Futsuhara Y, Matsuoka M, Yamaguchi J. 2001. slender rice, a constitutive gibberellin response mutant, is caused by a null mutation of the SLR1 gene, an ortholog of the height-regulating gene GAI/RGA/RHT/D8. The Plant Cell, 13, 999–1010.

Khush G S. 2001. Green revolution: The way forward. Nature Reviews Genetics, 2, 815–822.

Kikuchi F, Futsuhara Y. 1997. Inheritance of morphological characters. 2. Inheritance of semi-dwarf. In: Matsuo T, Kumazawa K, Ishii R, Ishihara K, Hirata H, eds., Science of the Rice Plant. vol 3. Food and Agricultural Policy Research Center, Tokyo, Japan. pp. 309–317.

Monna L, Kitazawa N, Yoshino R, Suzuki J, Masuda H, Maehara Y, Tanji M, Sato M, Nasu S, Minobe Y. 2002. Positional cloning of rice semidwarfing gene, sd-1: Rice “green revolution gene” encodes a mutant enzyme involved in gibberellins synthesis. DNA Research, 9, 11–17.

Murase K, Hirano Y, Sun T P, Hakoshima T. 2008. Gibberellin-induced DELLA recognition by the gibberellin receptor GID1. Nature, 456, 459–464.

Nishimura A, Aichi I, Matsuoka M. 2006. A protocol for Agrobacterium-mediated transformation in rice. Nature Protocol, 1, 2796–2802.

Peng J, Carol P, Richards D E, King K E, Cowling R J, Murphy G P, Harberd N P. 1997. The Arabidopsis GAI gene defines a signaling pathway that negatively regulates gibberellin responses. Genes & Development, 11, 3194–3205.

Peng J, Richards D E, Hartley N M, Murphy G P, Devos K M, Flintham J E, Beales J, Fish L J, Worland A J, Pelica F, Sudhakar D, Christou P, Snape J W, Gale M D, Harberd N P. 1999. ‘Green revolution’ genes encode mutant gibberellin response modulators. Nature, 400, 256–261.

Richards D E, King K E, Ait-ali T, Harberd N P. 2001. How gibberellins regulates plant growth and development: A molecular genetic analysis of gibberellins signaling. Annual Review Plant Physiology and Plant Molecular Biology, 52, 67–88.

Sakamoto T, Miura K, Itoh H, Tatsumi T, Ueguchitanaka M, Ishiyama K. 2004. An overview of gibberellin metabolism enzyme genes and their related mutants in rice. Plant Physiology, 134, 1642–1653.

Sanguinetti C J, Dias N E, Simpson A J G. 1994. Rapid silver staining and recover of PCR products separated on polyacrylamide gels. Biotechniques, 17, 915–919.

Sasaki A, Ashikari M, Ueguchi-Tanaka M, Itoh H, Nishimura A, Swapan D, Ishiyama K, Saito T, Kobayashi M, Khush G S, Kitano H, Matsuoka M. 2002. Green revolution: A mutant gibberellin-synthesis gene in rice. Nature, 416, 701–702.

Sasaki A, Itoh H, Gomi K, Ueguchi-Tanaka M, Ishiyama K, Kobayashi M, Jeong D H, An G, Kitano H, Ashikari M, Matsuoka M. 2003. Accumulation of phosphorylated repressor for gibberellins signaling in an F-box mutant. Science, 299, 1896–1898.

Spielmeyer W, Ellis M, Chandler P. 2002. Semidwarf (sd-1), “green revolution” rice, contains a defective gibberellin 20-oxidase gene. Proceedings of the National Academy of Sciences of the United States of America, 99, 9043–9048.

Sun T P, Gubler F. 2004. Molecular mechanism of gibberellin signalingin plants. Annual Review of Plant Biology, 55, 197–223.

Takeda K. 1977. Internode elongation and dwarfism in some graminaeous plants. Gamma Field Symposia, 16, 1–18.

Ueguchi-Tanaka M, Ashikari M, Nakajima M, Itoh H, Katoh E, Kobayashi M, Chow T Y, Hsing Y I, Kitano H, Yamaguchi I, Matsuoka M. 2005. GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin. Nature, 437, 693–698.

Ueguchi-Tanaka M, Nakajima M, Katoh E, Ohmiya H, Asano K, Saji S, Hongyu X, Ashikari M, Kitano H, Yamaguchi I, Matsuoka M. 2007. Molecular interactions of a soluble gibberellin receptor, GID1, with a rice DELLA protein, SLR1, and gibberellin. The Plant Cell, 19, 2140–2155.

Willige B C, Ghosh S, Nill C, Zourelidou M, Dohmann E M, Maier A, Schwechheimer C. 2007. The DELLA domain of GA INSENSITIVE mediates the interaction with the GA INSENSITIVE DWARF1A gibberellin receptor of Arabidopsis. The Plant Cell, 19, 1209–1220.

Yamaguchi S. 2008. Gibberellin metabolism and its regulation. Annual Review of Plant Biology, 59, 225–251.

Zhang Y H, Zhang S B, Lin J, Wang Y J, Fang X W. 2014. Research progress on cloning and functional analysis of plant height in rice (Oryza sativa L.). Chinese Agricultural Science Bulletin, 30, 1–7. (in Chinese)

Identification of a novel gain-of-function mutant allele, slr1-d5, of rice DELLA protein

Identification of a novel gain-of-function mutant allele, slr1-d5, of rice DELLA protein

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics