Effects of low ambient temperatures and dietary vitamin C supplementation on pulmonary vascular remodeling and hypoxic gene expression of 21-d-old broilers

doi:10.1016/S2095-3119(14)60968-0

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (1): 183-190.DOI: 10.1016/S2095-3119(14)60968-0

Effects of low ambient temperatures and dietary vitamin C supplementation on pulmonary vascular remodeling and hypoxic gene expression of 21-d-old broilers

ZENG Qiu-feng, YANG Xia, ZHENG Ping, ZHANG Ke-ying, LUO Yu-heng, DING Xue-mei, BAI Shi-ping, WANG Jian-ping, XUAN Yue, SU Zhuo-wei

Key Laboratory for Animal Disease Resistance Nutrition, Ministry of Education/Institute of Animal Nutrition, Sichuan Agricultural University,Ya’an 625014, P.R.China

收稿日期:2014-11-07 出版日期:2016-01-08 发布日期:2016-01-11
通讯作者: ZENG Qiu-feng, Mobile: +86-13778765040,Fax: +86-835-2885065, E-mail: zqf@sicau.edu.cn
作者简介:These authors contributed equally to this study
基金资助:
This research was supported by grants from the National Natural Science Foundation of China (31101733).

Effects of low ambient temperatures and dietary vitamin C supplementation on pulmonary vascular remodeling and hypoxic gene expression of 21-d-old broilers

ZENG Qiu-feng, YANG Xia, ZHENG Ping, ZHANG Ke-ying, LUO Yu-heng, DING Xue-mei, BAI Shi-ping, WANG Jian-ping, XUAN Yue, SU Zhuo-wei

Key Laboratory for Animal Disease Resistance Nutrition, Ministry of Education/Institute of Animal Nutrition, Sichuan Agricultural University,Ya’an 625014, P.R.China

Received:2014-11-07 Online:2016-01-08 Published:2016-01-11
Contact: ZENG Qiu-feng, Mobile: +86-13778765040,Fax: +86-835-2885065, E-mail: zqf@sicau.edu.cn
About author:These authors contributed equally to this study
Supported by:
This research was supported by grants from the National Natural Science Foundation of China (31101733).

摘要/Abstract

摘要： The objective of this study was to evaluate the effects of low ambient temperature (LAT) and dietary vitamin C (VC) supplementation on pulmonary vascular remodeling (PVR) and the relative expression of hypoxia inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and its receptor 2 (VEGFR-2) mRNA of lungs in 21-d-old broilers. 400 1-d-old male Cobb broilers were assigned randomly to 4 treatments as follows for 21 d: 1) LAT and a basal diet; 2) LAT and a basal diet supplemented with 1 000 mg kg–1 VC (LAT+VC); 3) normal ambient temperature (NAT) and a basal diet; 4) NAT and a basal diet supplemented with 1 000 mg kg–1 VC (NAT+VC). Each treatment was composed of 10 replicates of 10 birds per replicate. Samples of lung were collected after the broilers were killed at d 21. LAT increased the ratio of vessel wall area to vessel total area (WA/TA, %) and mean media thickness in pulmonary arterioles (mMTPA, %) (P<0.05). Dietary VC supplementation decreased mMTPA (P<0.05), but had no effect on the WA/TA. LAT increased (P<0.05) the relative mRNA expression of HIF-1α, VEGF and VEGFR-2, while adding VC to the diet could decrease (P<0.05) their relative mRNA expression. A significant positive correlation existed between the level of VEGF mRNA expression and the value of WA/WT (P<0.05) or mMTPA (P<0.05). These results suggested LAT resulted in pulmonary vascular remodeling, and the increase of HIF-1α, VEGF and VEGFR-2 mRNA expression, and dietary VC supplementation can alleviate pulmonary vascular remodeling in broiler by affecting these gene expression.

关键词: broiler , hypoxia inducible factor-1&alpha, low ambient temperature , pulmonary vascular remodeling , vitamin C

Abstract: The objective of this study was to evaluate the effects of low ambient temperature (LAT) and dietary vitamin C (VC) supplementation on pulmonary vascular remodeling (PVR) and the relative expression of hypoxia inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and its receptor 2 (VEGFR-2) mRNA of lungs in 21-d-old broilers. 400 1-d-old male Cobb broilers were assigned randomly to 4 treatments as follows for 21 d: 1) LAT and a basal diet; 2) LAT and a basal diet supplemented with 1 000 mg kg–1 VC (LAT+VC); 3) normal ambient temperature (NAT) and a basal diet; 4) NAT and a basal diet supplemented with 1 000 mg kg–1 VC (NAT+VC). Each treatment was composed of 10 replicates of 10 birds per replicate. Samples of lung were collected after the broilers were killed at d 21. LAT increased the ratio of vessel wall area to vessel total area (WA/TA, %) and mean media thickness in pulmonary arterioles (mMTPA, %) (P<0.05). Dietary VC supplementation decreased mMTPA (P<0.05), but had no effect on the WA/TA. LAT increased (P<0.05) the relative mRNA expression of HIF-1α, VEGF and VEGFR-2, while adding VC to the diet could decrease (P<0.05) their relative mRNA expression. A significant positive correlation existed between the level of VEGF mRNA expression and the value of WA/WT (P<0.05) or mMTPA (P<0.05). These results suggested LAT resulted in pulmonary vascular remodeling, and the increase of HIF-1α, VEGF and VEGFR-2 mRNA expression, and dietary VC supplementation can alleviate pulmonary vascular remodeling in broiler by affecting these gene expression.

Key words: broiler , hypoxia inducible factor-1&alpha, low ambient temperature , pulmonary vascular remodeling , vitamin C

ZENG Qiu-feng, YANG Xia, ZHENG Ping, ZHANG Ke-ying, LUO Yu-heng, DING Xue-mei, BAI Shi-ping, WANG Jian-ping, XUAN Yue, SU Zhuo-wei. Effects of low ambient temperatures and dietary vitamin C supplementation on pulmonary vascular remodeling and hypoxic gene expression of 21-d-old broilers[J]. Journal of Integrative Agriculture, 2016, 15(1): 183-190.

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Effects of low ambient temperatures and dietary vitamin C supplementation on pulmonary vascular remodeling and hypoxic gene expression of 21-d-old broilers

Effects of low ambient temperatures and dietary vitamin C supplementation on pulmonary vascular remodeling and hypoxic gene expression of 21-d-old broilers

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