Inoculation with chlamydospores of Trichoderma asperellum SM- 12F1 accelerated arsenic volatilization and influenced arsenic availability in soils

doi:10.1016/S2095-3119(14)60772-3

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (2): 389-397.DOI: 10.1016/S2095-3119(14)60772-3

Inoculation with chlamydospores of Trichoderma asperellum SM- 12F1 accelerated arsenic volatilization and influenced arsenic availability in soils

WANG Xiu-rong, SU Shi-ming, ZENG Xi-bai, BAI Ling-yu, LI Lian-fang, DUAN Ran, WANG Ya-nan, WU Cui-xia

Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Environment, Ministry of Agriculture, Beijing 100081, P.R.China

收稿日期:2013-11-30 出版日期:2015-02-01 发布日期:2015-02-11
通讯作者: ZENG Xi-bai,Tel: +86-10-82105612, E-mail: zengxibai@caas.cn
作者简介:WANG Xiu-rong, Tel: +86-10-82106009, E-mail: 124922870wxr@ sina.com; SU Shi-ming, Tel: +86-10-82106009, E-mail: sushiming@caas.cn; * These authors contributed equally to this study.
基金资助:
the financial support from the National Natural Science Foundation of China (41101296), and the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2012BAD14B02).

Inoculation with chlamydospores of Trichoderma asperellum SM- 12F1 accelerated arsenic volatilization and influenced arsenic availability in soils

WANG Xiu-rong, SU Shi-ming, ZENG Xi-bai, BAI Ling-yu, LI Lian-fang, DUAN Ran, WANG Ya-nan, WU Cui-xia

Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Environment, Ministry of Agriculture, Beijing 100081, P.R.China

Received:2013-11-30 Online:2015-02-01 Published:2015-02-11
Contact: ZENG Xi-bai,Tel: +86-10-82105612, E-mail: zengxibai@caas.cn
About author:WANG Xiu-rong, Tel: +86-10-82106009, E-mail: 124922870wxr@ sina.com; SU Shi-ming, Tel: +86-10-82106009, E-mail: sushiming@caas.cn; * These authors contributed equally to this study.
Supported by:
the financial support from the National Natural Science Foundation of China (41101296), and the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2012BAD14B02).

摘要/Abstract

摘要： Fungi capable of arsenic (As) accumulation and volatilization are hoped to tackle As-contaminated environment in the future. However, little data is available regarding their performances in field soils. In this study, the chlamydospores of Trichoderma asperellum SM-12F1 capable of As resistance, accumulation, and volatilization were inoculated into As-contaminated Chenzhou (CZ) and Shimen (SM) soils, and subsequently As volatilization and availability were assessed. The results indicated that T. asperellum SM-12F1 could reproduce well in As-contaminated soils. After cultivated for 42 days, the colony forming units (cfu) of T. asperellum SM-12F1 in CZ and SM soils reached 1010–1011 cfu g–1 fresh soil when inoculated at a rate of 5.0%. Inoculation with chlamydospores of T. asperellum SM-12F1 could significantly accelerate As volatilization from soils. The contents of volatilized As from CZ and SM soils after being inoculated with chlamydospores at a rate of 5.0% for 42 days were 2.0 and 0.6 μg kg–1, respectively, which were about 27.5 and 2.5 times higher than their corresponding controls of no inoculation (CZ, 0.1 μg kg–1; SM, 0.3 μg kg–1). Furthermore, the available As content in SM soils was decreased by 23.7%, and that in CZ soils increased by 3.3% compared with their corresponding controls. Further studies showed that soil pH values significantly decreased as a function of cultivation time or the inoculation level of chlamydospores. The pH values in CZ and SM soils after being inoculated with 5.0% of chlamydospores for 42 days were 6.04 and 6.02, respectively, which were lowered by 0.34 and 1.21 compared with their corresponding controls (CZ, 6.38; SM, 7.23). The changes in soil pH and As-binding fractions after inoculation might be responsible for the changes in As availability. These observations could shed light on the future remediation of As-contaminated soils using fungi.

关键词: arsenic , volatilization , availability , soil , chlamydospores , T. asperellum SM-12F1

Abstract: Fungi capable of arsenic (As) accumulation and volatilization are hoped to tackle As-contaminated environment in the future. However, little data is available regarding their performances in field soils. In this study, the chlamydospores of Trichoderma asperellum SM-12F1 capable of As resistance, accumulation, and volatilization were inoculated into As-contaminated Chenzhou (CZ) and Shimen (SM) soils, and subsequently As volatilization and availability were assessed. The results indicated that T. asperellum SM-12F1 could reproduce well in As-contaminated soils. After cultivated for 42 days, the colony forming units (cfu) of T. asperellum SM-12F1 in CZ and SM soils reached 1010–1011 cfu g–1 fresh soil when inoculated at a rate of 5.0%. Inoculation with chlamydospores of T. asperellum SM-12F1 could significantly accelerate As volatilization from soils. The contents of volatilized As from CZ and SM soils after being inoculated with chlamydospores at a rate of 5.0% for 42 days were 2.0 and 0.6 μg kg–1, respectively, which were about 27.5 and 2.5 times higher than their corresponding controls of no inoculation (CZ, 0.1 μg kg–1; SM, 0.3 μg kg–1). Furthermore, the available As content in SM soils was decreased by 23.7%, and that in CZ soils increased by 3.3% compared with their corresponding controls. Further studies showed that soil pH values significantly decreased as a function of cultivation time or the inoculation level of chlamydospores. The pH values in CZ and SM soils after being inoculated with 5.0% of chlamydospores for 42 days were 6.04 and 6.02, respectively, which were lowered by 0.34 and 1.21 compared with their corresponding controls (CZ, 6.38; SM, 7.23). The changes in soil pH and As-binding fractions after inoculation might be responsible for the changes in As availability. These observations could shed light on the future remediation of As-contaminated soils using fungi.

Key words: arsenic , volatilization , availability , soil , chlamydospores , T. asperellum SM-12F1

WANG Xiu-rong, SU Shi-ming, ZENG Xi-bai, BAI Ling-yu, LI Lian-fang, DUAN Ran, WANG Ya-nan, WU Cui-xia. Inoculation with chlamydospores of Trichoderma asperellum SM- 12F1 accelerated arsenic volatilization and influenced arsenic availability in soils[J]. Journal of Integrative Agriculture, 2015, 14(2): 389-397.

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Inoculation with chlamydospores of Trichoderma asperellum SM- 12F1 accelerated arsenic volatilization and influenced arsenic availability in soils

Inoculation with chlamydospores of Trichoderma asperellum SM- 12F1 accelerated arsenic volatilization and influenced arsenic availability in soils

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