Over-Expression of ScMnSOD, a SOD Gene Derived from Jojoba, Improve Drought Tolerance in Arabidopsis

doi:10.1016/S2095-3119(13)60404-9

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (10): 1722-1730.DOI: 10.1016/S2095-3119(13)60404-9

Over-Expression of ScMnSOD, a SOD Gene Derived from Jojoba, Improve Drought Tolerance in Arabidopsis

LIU Xiao-fei, SUN Wei-min, LI Ze-qin, BAI Rui-xue, LI Jing-xiao, SHI Zi-han, GENG Hongwei, ZHENG Ying, ZHANG Jun , ZHANG Gen-fa

1 Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing 100875, P.R.China
2 College of Life Sciences, Beijing Normal University, Beijing 100875, P.R.China
3 COE For Neuroscience, Departments of Anesthesiology, Biomedical Sciences, Texas Tech University Health Sciences Center, Texas
79905, USA

收稿日期:2012-11-10 出版日期:2013-10-01 发布日期:2013-10-01
通讯作者: Correspondence ZHANG Gen-fa, Tel: +86-10-58809453, Fax: +86-10-58807720, E-mail: gfzh@bnu.edu.cn
作者简介:LIU Xiao-fei, E-mail: lxf541@163.com
基金资助:
This work was kindly supported by the National Natural Sciences Foundation of China (30970286, 31270362 and 31070289).

Over-Expression of ScMnSOD, a SOD Gene Derived from Jojoba, Improve Drought Tolerance in Arabidopsis

LIU Xiao-fei, SUN Wei-min, LI Ze-qin, BAI Rui-xue, LI Jing-xiao, SHI Zi-han, GENG Hongwei, ZHENG Ying, ZHANG Jun , ZHANG Gen-fa

1 Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, Beijing 100875, P.R.China
2 College of Life Sciences, Beijing Normal University, Beijing 100875, P.R.China
3 COE For Neuroscience, Departments of Anesthesiology, Biomedical Sciences, Texas Tech University Health Sciences Center, Texas
79905, USA

Received:2012-11-10 Online:2013-10-01 Published:2013-10-01
Contact: Correspondence ZHANG Gen-fa, Tel: +86-10-58809453, Fax: +86-10-58807720, E-mail: gfzh@bnu.edu.cn
About author:LIU Xiao-fei, E-mail: lxf541@163.com
Supported by:
This work was kindly supported by the National Natural Sciences Foundation of China (30970286, 31270362 and 31070289).

摘要/Abstract

摘要： Jojoba (Simmondsia chinensis) is mainly distributed in desert, and the molecular mechanisms of jojoba in response to abiotic stress still remain elusive. In this paper, we cloned and characterized a SOD gene from jojoba named as ScMnSOD, and introduced into Arabidopsis to investigate its functions of responding to drought stress. The transgenic Arabidopsis showed an improvement in drought tolerance. Moreover, under a water deficit condition, the accumulation of reactive oxygen species (ROS) was remarkably decreased in the transgenic lines compared to the WT. Furthermore, the ScMnSOD promoter was cloned to the 5´-upstream of GUS coding region in a binary vector, and introduced into Arabidopsis. And results showed that ScMnSOD expression can be induced by drought, salt, ABA, and low temperature. In conclusion, ScMnSOD plays an important role in drought tolerance which is, at least partially, attributed to its role in ROS detoxification.

关键词: drought , jojoba , promoter , ROS , ScMnSOD , stress tolerance

Abstract: Jojoba (Simmondsia chinensis) is mainly distributed in desert, and the molecular mechanisms of jojoba in response to abiotic stress still remain elusive. In this paper, we cloned and characterized a SOD gene from jojoba named as ScMnSOD, and introduced into Arabidopsis to investigate its functions of responding to drought stress. The transgenic Arabidopsis showed an improvement in drought tolerance. Moreover, under a water deficit condition, the accumulation of reactive oxygen species (ROS) was remarkably decreased in the transgenic lines compared to the WT. Furthermore, the ScMnSOD promoter was cloned to the 5´-upstream of GUS coding region in a binary vector, and introduced into Arabidopsis. And results showed that ScMnSOD expression can be induced by drought, salt, ABA, and low temperature. In conclusion, ScMnSOD plays an important role in drought tolerance which is, at least partially, attributed to its role in ROS detoxification.

Key words: drought , jojoba , promoter , ROS , ScMnSOD , stress tolerance

LIU Xiao-fei, SUN Wei-min, LI Ze-qin, BAI Rui-xue, LI Jing-xiao, SHI Zi-han, GENG Hongwei, ZHENG Ying, ZHANG Jun , ZHANG Gen-fa. Over-Expression of ScMnSOD, a SOD Gene Derived from Jojoba, Improve Drought Tolerance in Arabidopsis[J]. Journal of Integrative Agriculture, 2013, 12(10): 1722-1730.

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Over-Expression of ScMnSOD, a SOD Gene Derived from Jojoba, Improve Drought Tolerance in Arabidopsis

Over-Expression of ScMnSOD, a SOD Gene Derived from Jojoba, Improve Drought Tolerance in Arabidopsis

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