Conophylline Promotes the Proliferation of Immortalized Mesenchymal Stem Cells Derived from Fetal Porcine Pancreas (iPMSCs)

doi:10.1016/S2095-3119(13)60286-5

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (4): 678-686.DOI: 10.1016/S2095-3119(13)60286-5

Conophylline Promotes the Proliferation of Immortalized Mesenchymal Stem Cells Derived from Fetal Porcine Pancreas (iPMSCs)

ZHANG Hui-ru, LI Dan, CAO Hui, LÜ Xiao, CHU Yuan-kui, BAI Yao-fu, JIN Ya-ping, PENG

1.College of Bioengineering, Henan University of Technology, Zhengzhou 450001, P.R.China
2.College of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering & Technology/Key Lab for Animal Biotechnology of Agriculture Ministry of China, Northwest A&F University, Yangling 712100, P.R.China

收稿日期:2011-11-08 出版日期:2013-04-01 发布日期:2013-04-07
通讯作者: Correspondence HUA Jin-lian, Tel: +86-29-87080068, Fax: +86-29-87080068, E-mail: jlhua2003@126.com; PENG Sha, E-mail: pengsha123@yahoo.com.cn
基金资助:
This work was supported by the grants from the National Natural Science Foundation of China (31272518, 31101775, 30972097), the Doctoral Fund of Ministry of Education of China (20100204120020), the Program for New Century Excellent Talents of State Ministry of Education (NCET-09-0654), and the Scientific Research Program of Shaanxi Province (2011K02-06, 2008K02-05), the Scientific Research Program of Shaanxi Province, China (2011K02-06), the Fundamental Research Funds for the Central Universities, China (QN2011012), and the Graduate Education Innovation Projects of Henan University of Technology, China (11YJCX45).

Conophylline Promotes the Proliferation of Immortalized Mesenchymal Stem Cells Derived from Fetal Porcine Pancreas (iPMSCs)

ZHANG Hui-ru, LI Dan, CAO Hui, LÜ Xiao, CHU Yuan-kui, BAI Yao-fu, JIN Ya-ping, PENG

1.College of Bioengineering, Henan University of Technology, Zhengzhou 450001, P.R.China
2.College of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering & Technology/Key Lab for Animal Biotechnology of Agriculture Ministry of China, Northwest A&F University, Yangling 712100, P.R.China

Received:2011-11-08 Online:2013-04-01 Published:2013-04-07
Contact: Correspondence HUA Jin-lian, Tel: +86-29-87080068, Fax: +86-29-87080068, E-mail: jlhua2003@126.com; PENG Sha, E-mail: pengsha123@yahoo.com.cn
Supported by:
This work was supported by the grants from the National Natural Science Foundation of China (31272518, 31101775, 30972097), the Doctoral Fund of Ministry of Education of China (20100204120020), the Program for New Century Excellent Talents of State Ministry of Education (NCET-09-0654), and the Scientific Research Program of Shaanxi Province (2011K02-06, 2008K02-05), the Scientific Research Program of Shaanxi Province, China (2011K02-06), the Fundamental Research Funds for the Central Universities, China (QN2011012), and the Graduate Education Innovation Projects of Henan University of Technology, China (11YJCX45).

摘要/Abstract

摘要： Conophylline, is a bis (indole) alkaloid consisting of two pentacyclic aspidosperma skeletons, isolated from Tabernaemontana divaricata, which has been found to induce b-cell differentiation in rat pancreatic acinar carcinoma cells and in cultured rat pancreatic tissue. However, the precise role of conophylline in the growth and survival of immortalized pancreatic mesenchymal stem cells (iPMSCs) derived from fetal porcine pancreas were not understood at present. To determine whether this molecule is involved in controlling the proliferation of iPMSCs, we examined the effects of conophylline on iPMSCs. We found that conophylline can robustly stimulate iPMSCs proliferation, even promote their potential differentiation into islet-like clusters analyzed by cell counting, morphology, RT-PCR and real-time PCR, Western blotting, glucose-stimulated insulin release and insulin content analysis. The effects of conophylline were inhibited by LY294002, which is the inhibitor of the PI3K pathway. These results suggest that conophylline plays a key role in the regulation of cell mass proliferation, maintenance of the undifferentiated state of iPMSCs and also promotes iPMSCs differentiated into insulin-producing cells.

关键词: immortalized pancreatic mesenchymal stem cells (iPMSCs) , conophylline , PI3K , insulin-producing cells

Abstract: Conophylline, is a bis (indole) alkaloid consisting of two pentacyclic aspidosperma skeletons, isolated from Tabernaemontana divaricata, which has been found to induce b-cell differentiation in rat pancreatic acinar carcinoma cells and in cultured rat pancreatic tissue. However, the precise role of conophylline in the growth and survival of immortalized pancreatic mesenchymal stem cells (iPMSCs) derived from fetal porcine pancreas were not understood at present. To determine whether this molecule is involved in controlling the proliferation of iPMSCs, we examined the effects of conophylline on iPMSCs. We found that conophylline can robustly stimulate iPMSCs proliferation, even promote their potential differentiation into islet-like clusters analyzed by cell counting, morphology, RT-PCR and real-time PCR, Western blotting, glucose-stimulated insulin release and insulin content analysis. The effects of conophylline were inhibited by LY294002, which is the inhibitor of the PI3K pathway. These results suggest that conophylline plays a key role in the regulation of cell mass proliferation, maintenance of the undifferentiated state of iPMSCs and also promotes iPMSCs differentiated into insulin-producing cells.

Key words: immortalized pancreatic mesenchymal stem cells (iPMSCs) , conophylline , PI3K , insulin-producing cells

ZHANG Hui-ru, LI Dan, CAO Hui, Lü Xiao, CHU Yuan-kui, BAI Yao-fu, JIN Ya-ping, PENG. Conophylline Promotes the Proliferation of Immortalized Mesenchymal Stem Cells Derived from Fetal Porcine Pancreas (iPMSCs)[J]. Journal of Integrative Agriculture, 2013, 12(4): 678-686.

ZHANG Hui-ru, LI Dan, CAO Hui, Lü Xiao, CHU Yuan-kui, BAI Yao-fu, JIN Ya-ping, PENG . Conophylline Promotes the Proliferation of Immortalized Mesenchymal Stem Cells Derived from Fetal Porcine Pancreas (iPMSCs)[J]. Journal of Integrative Agriculture, 2013, 12(4): 678-686.

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Conophylline Promotes the Proliferation of Immortalized Mesenchymal Stem Cells Derived from Fetal Porcine Pancreas (iPMSCs)

Conophylline Promotes the Proliferation of Immortalized Mesenchymal Stem Cells Derived from Fetal Porcine Pancreas (iPMSCs)

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