Development of Transgenic Glyphosate-Resistant Rice with G6 GeneEncoding 5-Enolpyruvylshikimate-3-Phosphate Synthase

doi:10.1016/S1671-2927(11)60123-5

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (9): 1307-1312.DOI: 10.1016/S1671-2927(11)60123-5

• 论文 • 下一篇

Development of Transgenic Glyphosate-Resistant Rice with G6 GeneEncoding 5-Enolpyruvylshikimate-3-Phosphate Synthase

ZHAO Te, LIN Chao-yang , SHEN Zhi-cheng

State Key Laboratory of Rice Biology and Institute of Insect Sciences, Zhejiang University

收稿日期:2011-06-02 出版日期:2011-09-01 发布日期:2011-09-09
通讯作者: Correspondence SHEN Zhi-cheng, Professor, Tel/Fax: +86-571-86971273, E-mail: zcshen@zju.edu.cn
作者简介:ZHAO Te, Ph D, Mobile: 15838136601, E-mail: tezhao@126.com

Development of Transgenic Glyphosate-Resistant Rice with G6 GeneEncoding 5-Enolpyruvylshikimate-3-Phosphate Synthase

ZHAO Te, LIN Chao-yang , SHEN Zhi-cheng

State Key Laboratory of Rice Biology and Institute of Insect Sciences, Zhejiang University

Received:2011-06-02 Online:2011-09-01 Published:2011-09-09
Contact: Correspondence SHEN Zhi-cheng, Professor, Tel/Fax: +86-571-86971273, E-mail: zcshen@zju.edu.cn
About author:ZHAO Te, Ph D, Mobile: 15838136601, E-mail: tezhao@126.com

摘要/Abstract

摘要： Glyphosate-resistant crops have been a huge economic success for genetic engineering. The creating of new glyphosateresistantplants would increase the available choices for planting and lower the price of genetically modified crop seeds.A novel G6 gene from Pseudomonas putida that encoded 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) waspreviously isolated. The G6 gene was transfected into rice via Agrobacterium-mediated transformation. The transgenicrice obtained was confirmed by PCR, Southern, and Western blots. The lab experiment and field trials further confirmedthat the transgenic rice can survive glyphosate spraying at a dose of 8 g L-1. In contrast, conventional rice was killed ata weed control glyphosate spray dose of 1 g L-1. Altogether, the present study showed that the G6 gene works well in ricein vivo for glyphosate-resistance.

关键词: transgenic rice, glyphosate-resistance, EPSPS

Abstract: Glyphosate-resistant crops have been a huge economic success for genetic engineering. The creating of new glyphosateresistantplants would increase the available choices for planting and lower the price of genetically modified crop seeds.A novel G6 gene from Pseudomonas putida that encoded 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) waspreviously isolated. The G6 gene was transfected into rice via Agrobacterium-mediated transformation. The transgenicrice obtained was confirmed by PCR, Southern, and Western blots. The lab experiment and field trials further confirmedthat the transgenic rice can survive glyphosate spraying at a dose of 8 g L-1. In contrast, conventional rice was killed ata weed control glyphosate spray dose of 1 g L-1. Altogether, the present study showed that the G6 gene works well in ricein vivo for glyphosate-resistance.

Key words: transgenic rice, glyphosate-resistance, EPSPS

ZHAO Te, LIN Chao-yang , SHEN Zhi-cheng. Development of Transgenic Glyphosate-Resistant Rice with G6 GeneEncoding 5-Enolpyruvylshikimate-3-Phosphate Synthase[J]. Journal of Integrative Agriculture, 2011, 10(9): 1307-1312.

参考文献

[1]Battraw M J, Hall T C. 1990. Histochemical analysis of CaMV35S promoter-β-glucuronidase gene expression in transgenicrice plants. Plant Molecular Biology, 15, 527-538.

[2]Cao M X, Huang J Q, Wei Z M, Yao Q H, Wan C Z, Lu J A.2004. Engineering higher yield and herbicide resistance inrice by agrobacterium-mediated multiple gene transformation.Crop Science, 44, 2206-2213.

[3]Christensen A H, Sharrock R A, Quail P H. 1992. Maizepolyubiquitin genes: structure, thermal perturbation ofexpression and transcript splicing, and promoter activityfollowing transfer to protoplasts by electroporation. PlantMolecular Biology, 18, 675-689.

[4]Cornejo M J, Luth D, Blankenship K M, Anderson O D, BlechlA E. 1993. Activity of a maize ubiquitin promoter in transgenicrice. Plant Molecular Biology, 23, 567-581.

[5]Della-Cioppa G, Bauer S C, Klein B K, Shah D M, Fraley R T,Kishore G M. 1986. Translocation of the precursor of 5-enolpyruvylshikimate 3-phosphate synthase intochloroplasts of higher plants in vitro. Procedings of theNational Academy of Science of the USA, 83, 6873-6877.

[6]Dill G M. 2005. Glyphosate-resistant crops: History, statusand future. Pest Management Science, 61, 219-224.

[7]Gruys K J, Marzabadi M R, Pansegrau P D, Sikorski J A. 1993.Stead-ystate kinetic evaluation of the reverse reaction forEscherichia coli 5-enolpyruvylshikimate-3-phosphatesynthase. Archives of Biochemistry and Biophysics, 304, 345-351.

[8]Herman K M, Weaver L M. 1999. The shikimate pathway.Annual Review of Plant Physiology and Plant MolecularBiology, 50, 473-503.

[9]Howe A R, Gasser C S, Brown S M, Padgette S R, Hart J, ParkerG B, Fromm M E, Armstrong C L. 2002. Glyphosate as aselective agent for the production of fertile transgenic maize(Zea mays L.). Molecular Breeding, 10, 153-164.

[10]Hu T, Metz S, Chay C, Zhou H P, Biest N, Chen G, Cheng M,Feng X, Radionenko M, Lu F, Fry J. 2003. Agrobacteriummediatedlarge-scale transformation of wheat (Triticumaestivum L.) using glyphosate selection. Plant Cell Reports,21, 1010-1019.

[11]Kishore G M, Padgette S R, Fraley R T. 1992. History ofherbicide-tolerant crops, methods of development and currentstate of the art-emphasis on glyphosate tolerance. WeedTechnology, 6, 626-634.

[12]Lin C Y, Fang J, Xu X L, Zhao T, Cheng J A, Tu J M, Ye G, ShenZ C. 2008. A built-in strategy for containment of transgenicplants: creation of selectively terminable transgenic rice. PLoSONE, 3, e1818.McElroy D, Blowers A, Jenes B, Wu R. 1991. Construction ofexpression vectors based on the rice actin 1 (Act1) 5 regionfor use in monocot transformation. Molecular Genetics andGenomics, 231, 150-160.

[13]McElroy D, Zhang W, Wu R. 1990. Isolation of an efficient actinpromoter for use in rice transformation. The Plant Cell, 2,163-171.

[14]Padgette S R, Re D B, Gasser C S, Eichholtz D A, Frazier R B,Hironaka C M, Levine E B, Sha D M, Fraley R T, Kishore GM. 1991. Site-directed mutagenesis of a conserved region ofthe 5-enolpyruvylshikimate-3-phosphate synthase activesite. The Journal of Biological Chemistry, 266, 22364-22369.

[15]Schledzewski K, Mendel R. 1994. Quantitative transient geneexpression: comparison of the promoters for maizepolyubiquitin 1 rice actin 1, maize derivedEmu and CaMV35S in cells of barley, maize and tobacco. TransgenicResearch, 3, 249-255.

[16]Schmid J, Amrhein N. 1995. Molecular organization of theshikimate pathway in higher plants. Phytochemistry, 39, 737-749.

[17]Xu X, Kawasaki S, Fujimura T, Wang C. 2005. A protocol forhigh-throughput extraction of DNA from rice leaves. PlantMolecular Biology Reporter, 23, 291-295.

[18]Zhou M, Xu H L, Wei X L, Ye Z Q, Wei L P, Gong W M, WangY Q, Zhu Z. 2006. Identification of a glyphosate-resistantmutant of rice 5-enolpyruvylshikimate-3-phosphatesynthase using a directed evolution strategy. PlantPhysiology, 140, 184-195.

Development of Transgenic Glyphosate-Resistant Rice with G6 GeneEncoding 5-Enolpyruvylshikimate-3-Phosphate Synthase

Development of Transgenic Glyphosate-Resistant Rice with G6 GeneEncoding 5-Enolpyruvylshikimate-3-Phosphate Synthase

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 7

编辑推荐

Metrics

[1]	RONG Rui-juan, WU Peng-cheng, LAN Jin-ping, WEI Han-fu, WEI Jian, CHEN Hao, SHI Jia-nan, HAO Yu-jie, LIU Li-juan, DOU Shi-juan, LI Li-yun, WU Lin, LIU Si-qi, YIN Chang-cheng, LIU Guo-zhen. Western blot detection of PMI protein in transgenic rice[J]. Journal of Integrative Agriculture, 2016, 15(4): 726-734.
[2]	ZHANG Chun, FENG Li, HE Ting-ting, YANG Cai-hong, CHEN Guo-qi, TIAN Xing-shan. Investigating the mechanisms of glyphosate resistance in goosegrass (Eleusine indica) population from South China[J]. Journal of Integrative Agriculture, 2015, 14(5): 909-918.
[3]	JIN Yong-mei, MA Rui, YU Zhi-jing, WANG Ling, JIANG Wen-zhu, LIN Xiu-feng. Development of lepidopteran pest-resistant transgenic japonica rice harboring a synthetic cry2A* gene[J]. Journal of Integrative Agriculture, 2015, 14(3): 423-429.
[4]	QIANG Xiao-jing, YU Guo-hong, JIANG Lin-lin, SUN Lin-lin, ZHANG Shu-hui, LI Wei, CHENG Xian-guo. Thellungiella halophila ThPIP1 gene enhances the tolerance of the transgenic rice to salt stress[J]. Journal of Integrative Agriculture, 2015, 14(10): 1911-1922.
[5]	JIANG Xian-bin, HUANG Qian, LING Yan, CHEN Yu-chong, XIAO Guo-ying, HUANG Suo-sheng, WU Bi-qiu, HUANG Feng-kuan, CAI Jian-he, LONG Li-ping. Functional and numerical responses of Cyrtorhinus lividipennis to eggs of Nilaparvata lugens are not affected by genetically modified herbicide-tolerant rice[J]. Journal of Integrative Agriculture, 2015, 14(10): 2019-2026.
[6]	LU Zeng-bin, HAN Nai-shun, TIAN Jun-ce, PENG Yu-fa, HU Cui, GUO Yu-yuan, SHEN Zhicheng, YE Gong-yin. Transgenic cry1Ab/vip3H+epsps Rice with Insect and Herbicide Resistance Acted No Adverse Impacts on the Population Growth of a Non-Target Herbivore, the White-Backed Planthopper, Under Laboratory and Field Conditions[J]. Journal of Integrative Agriculture, 2014, 13(12): 2678-2689.
[7]	ZHAO Xiang-xiang, TANG Tang, LIU Fu-xia, LU Chang-li, HU Xiao-lan, JI Li-lian , LIU Qiaoquan. Unintended Changes in Genetically Modified Rice Expressing the Lysine-Rich Fusion Protein Gene Revealed by a Proteomics Approach[J]. Journal of Integrative Agriculture, 2013, 12(11): 2013-2021.