Effect ofAscorbic Acid and Silicium on Photosynthesis, Antioxidant Enzyme Activity, and Fatty Acid Contents in Canola Exposure to Salt Stress

doi:10.1016/S1671-2927(00)8694

摘要/Abstract

摘要： The effects of exogenous ascorbic acid and silicium on leaf fresh weigh, seed yield, photosynthesis, changes of the activities of major antioxidant enzymes, nitrate reductase activity, proline accumulation, chlorophyll content, and fatty acid composition were investigated in salt-stressed canola. A hydroponic pot experiment was conducted based on randomized complete block design, factorial arrangement was used with 16 combinations of salinity stress (0, 100, 200, and 300 mmol L-1 NaCl), ascorbic acid (0 and 30 mmol L-1), and silicium (2 and 4 mmol L-1 from potassium silicate) with three replicates. The results showed that salinity significantly decreased leaf area and leaf fresh weight, seed yield, photosynthesis, nitrate reductase activity, chlorophyll content, and seed protein percentage. Conversely, respiration, antioxidant enzymes activity, proline accumulation, and linolenic acid percentage increased due to salt stress. Ascorbic acid application improved photosynthesis and seed yield and mitigated antioxidant enzyme activity. In addition, nitrate reductase activity and chlorophyll a and b were positively affected by ascorbic acid. Regarding silicium application, that was found that leaf area, leaf fresh weight, seed yield and photosynthesis, ascorbate peroxidase activity, nitrate reductase activity, and chlorophyll content increased, while respiration decreased. Furthermore, silicium had not significant effect on antioxidant enzyme activity. In general, ascorbic acid and silicium were involved in the defensive mechanisms against salinity stress and it can be suggested that, ascorbic acid and silicium application had positive effect on canola growth under conditions of salt stress.

关键词: ascorbic acid , salt stress , canola , silicium

Abstract: The effects of exogenous ascorbic acid and silicium on leaf fresh weigh, seed yield, photosynthesis, changes of the activities of major antioxidant enzymes, nitrate reductase activity, proline accumulation, chlorophyll content, and fatty acid composition were investigated in salt-stressed canola. A hydroponic pot experiment was conducted based on randomized complete block design, factorial arrangement was used with 16 combinations of salinity stress (0, 100, 200, and 300 mmol L-1 NaCl), ascorbic acid (0 and 30 mmol L-1), and silicium (2 and 4 mmol L-1 from potassium silicate) with three replicates. The results showed that salinity significantly decreased leaf area and leaf fresh weight, seed yield, photosynthesis, nitrate reductase activity, chlorophyll content, and seed protein percentage. Conversely, respiration, antioxidant enzymes activity, proline accumulation, and linolenic acid percentage increased due to salt stress. Ascorbic acid application improved photosynthesis and seed yield and mitigated antioxidant enzyme activity. In addition, nitrate reductase activity and chlorophyll a and b were positively affected by ascorbic acid. Regarding silicium application, that was found that leaf area, leaf fresh weight, seed yield and photosynthesis, ascorbate peroxidase activity, nitrate reductase activity, and chlorophyll content increased, while respiration decreased. Furthermore, silicium had not significant effect on antioxidant enzyme activity. In general, ascorbic acid and silicium were involved in the defensive mechanisms against salinity stress and it can be suggested that, ascorbic acid and silicium application had positive effect on canola growth under conditions of salt stress.

Key words: ascorbic acid , salt stress , canola , silicium

Ahmad Bybordi. Effect ofAscorbic Acid and Silicium on Photosynthesis, Antioxidant Enzyme Activity, and Fatty Acid Contents in Canola Exposure to Salt Stress[J]. Journal of Integrative Agriculture, 2012, 12(10): 1610-1620.

参考文献

[1]Adatia M H, Besford R T. 1986. The effect of silicon oncucumber plants grown in recirculating nutrientsolution. Annals of Botany, 58, 343-351.

[2]Aery N C, Jagetiya B L. 2000. Effect of cobalt treatments ondry matter production of wheat and DTPA extractablecobalt content in soils. Communications in Soil Scienceand Plant Analysis, 31, 1275-1286.

[3]Agarie S, Agata W, Kubota F, Kaufman P B. 1992. Physiological roles of silicon in photosynthesis anddry matter production in rice plants. I. effects of siliconand shading treatments. Japanese Journal of CropScience, 61, 200-206.

[4]Ahmad P, Jhon R. 2005. Effect of salt stress on growth andbiochemical parameters of Pisum sativum L. Archivesof Agronomy and Soil Science, 51, 665-672.

[5]Al-aghabary K, Zhu Z, Qinhua S. 2004. Influence of siliconsupply on chlorophyll content, chlorophyll fluorescenceand antioxidative enzyme activities in tomato plants undersalt stress. Journal of Plant Nutrition, 27, 2101-2115.

[6]Anuradha S, Rao S S R. 2003.Application of brassinosteroidsto rice seeds (Oryza sativa L.) reduced the impact ofsalt stress on growth and improved photosyntheticpigment levels and nitrate reductase activity. PlantGrowth Regulation, 40, 29-32.

[7]Arnon D I. 1949. Copper enzymes in isolated chloroplasts,polyphennoloxidase in Beta vulgaris. Plant Physiology,24, 1-150.

[8]Asada K. 1999. The water-water cycle in chloroplasts:scavenging of active oxygen and dissipation of excessphotons. Annual Review of Plant Physiology andPlant Molecular Biology, 50, 601-639.

[9]Ashraf M, Karim F, Rasul E. 2002. Interactive effects ofgibberellic acid (GA3) and salt stress on growth, ionaccumulation and photosynthetic capacity of twospring wheat (Triticum aestivum L.) cultivars differingin salt tolerance. Plant Growth Regulation, 36, 49-59.

[10]Bates L S,Waldern R P, Teave I D. 1973. Rapid determinationof free proline for water stress studies. Plant and Soil,39, 205-207.

[11]Binzel M L, Hasegawa P M, Rhodes D, Handa S, HandaAK, Bressan R A. 1987. Solute accumulation in tobaccocells adapted to NaCl. Plant Physiology, 84, 1408-1415.

[12]Bolwell G P, Bindschedler LV, Blee KA, Butt V S, Davies DR, Gardner S L, Gerrish C, Minibayeva F. 2002. Theapoplastic oxidative burst in response to biotic stressin plants: a three-component system. Journal ofExperimental Botany, 53, 1367-1376.

[13]Buettner G R, Schafer F Q. 2004.Ascorbate as an antioxidantin vitamin C. In: Asard H, May J M, Smirnoff N, eds.,Functions and Biochemistry in Animals and Plants.Bios Scientific Publishers, Oxford. pp. 173-188.

[14]Cakmak I, Horst W. 1991. Affect of aluminium on lipidperoxidation, superoxide dismutase, catalase andperoxidase activities in root tip of soybean (Glysin max).Plant Physiology, 83, 463-468.

[15]Chen Z, Gallie D R. 2004. The ascorbic acid redox statecontrols guard cell signaling and stomatal movement.The Plant Cell, 16, 1143-1162.

[16]Datnoff L E, Snyder G H, Korndörfer G H. 2001. Silicon inAgriculture. Elsevier, Amsterdam.Davey M W, Mantagu M V, Dirk I, Maite S, Angelos K,Smirnoff N, Binenzie I J J, Strain J J D, Favell D, FletcherJ. 2000. Plant ascorbic: acid chemistry, function,metabolism, bioavailbility and effects of processing.Journal of the Science of Food and Agriculture, 80,825-850.

[17]Deren C W, Datnoff L E, Snyder G H, Martin F G. 1994.Silicon concentration, disease response, and yieldcomponents of rice genotypes grown on floodedorganic histosols. Crop Science, 34, 733-737.

[18]DeRidder B P, Salvucci M. 2007. Modulation pf Rubiscoactivase gene expression during heat stress in cotton(Gossyoium hirsutum L.) involves post-transcriptionalmechanisms. Plant Science, 172, 246-252.

[19]Dionisio-Sese M L, Tobita S. 2000. Effects of salinity onsodium content and photosynthetic responses of riceseedlings differing in salt tolerance. Journal of PlantPhysiology, 157, 54-58.

[20]Epstein E. 1994. The anomaly of silicon in plant biology.Proceedings of the National Academy of Sciences ofthe United States of America, 91, 11-17.

[21]Epstein E. 1999. Silicon. Annual Review of Plant Physiologyand Plant Molecular Biology, 50, 641-664.

[22]Fadzilla NM, Finch R P, Burdon R H. 1997. Salinity, oxidativestress and antioxidant responses in shoot cultures ofrice. Journal of Experimental Botany, 48, 325-331.

[23]Foyer C H, Halliwell B. 1976. The presence of glutathioneand glutathione reductase in chloroplasts: a proposedrole in ascorbic acid metabolism. Planta, 133, 21-25.

[24]Foyer C H, Noctor G. 2003. Redox sensing and signallingassociated with reactive oxygen in chloroplasts,peroxisomes and mitochondria. Plant Physiology, 119,355-364.

[25]Ghanati F, MoritaA, Yokota H. 2002. Induction of suberin andincrease of liginin content by excess boron in tobaccocell. Soil Science and Plant Nutrition, 48, 357-364.

[26]Giannopolitis C, Ries S. 1977. Superoxide dismutaseoccurrence in higher plant. Plant Physiology, 59, 309-314.

[27]Gong H, Zhu X, Chen K, Wang S, Zhang S. 2005. Siliconalleviates oxidative damage of wheat plants in potsunder drought. Plant Science, 169, 313-321.

[28]Gouia H, GhorbalMH, Touraine B. 1994. Effects of NaCl onflows of N and mineral ions and NO3-reductase ratewithin whole plants of salt-sensitive bean andsalttolerant cotton. Plant Physiology, 105, 1407-1418.

[29]Guo Z G , Liu H X, Tian F P, Zhang Z H, Wang S M. 2006.Effect of silicon on the morphology of shoots and rootsof alfalfa (Medicago sativa). Australian Journal ofExperimental Agriculture, 46, 1161-1166.

[30]Hamada A M. 1998. Effect of exogenously added ascorbicacid, thiamin or aspirin on photosynthesis and somerelated activities of drought-stressed wheat plants. In:Proceedings of the XIth International PhotosynthesisConference. Budapest, Hungary, August. pp. 17-22.

[31]Hasegawa P M, Bressan R A, Zhu J K, Bohnert H J. 2000.Plant cellular and molecular responses to high salinity.Annual Review of Plant Physiology and PlantMolecular Biology, 51, 463-499.

[32]Hussain S, Ismail S. 1994. Effect of salt and water stress ongrowth and biomass production in Helianthus annuus L. Pakistan Journal of Botany, 26, 127-138.

[33]Kasai K, Fukayama H, Uchida N, Mori N, Yasuda T,Nakamura C. 1998. Salinity tolerance in triticumaestivum, lophopyrum elongatum amphiploid and 5Edisomic addition line evaluated by NaCl effects onphotosynthesis and respiration. Cereal ResearchCommunications, 26, 281-287.

[34]Kefeli V I. 1981. Vitamins and some other representativesof non hormonal plant growth regulators. AppliedBiochemistry and Microbiology, 17, 5-15.

[35]Ketchum R E B, Warren R C, Klima L J, Lopez-Gutierrez F,Nabors M W. 1991. The mechanism and regulation ofproline accumulation in suspension cultures of thehalophytic grass Distichlis spicata L. Journal of PlantPhysiology, 137, 368-374.

[36]Khan M G, Silberbush M, lips S H. 1995. Physiologicalstudies of salinity and nitrogen interaction in alfalfaplants. III. Nitrate reductase activity. Journal of PlantNutrition, 18, 2495-2500.

[37]Klepper L, Flesher D, Hageman R H. 1971. Generation ofreduced nicotinamide adenine dinucleotide for nitratereduction in green leaves. Plant Physiology, 20, 580-590.

[38]Liang Y C, Chen Q, Liu Q, Zhang W, Ding R. 2003.Exogenous silicon (Si) increases antioxidant enzymeactivity and reduces lipid peroxidation in roots of saltstressed.Plant and Soil, 255, 85-91.

[39]Liang Y C, Shen Q R, Shen Z Q, Ma T S. 1996. Effects ofsilicon on salinity tolerance of two barley cultivars.Journal of Plant Nutrition, 19, 173-183.

[40]Liang Y C, Sun W, Zhu Y G, Christie P. 2007. Mechanisms ofsilicon mediated alleviation of abiotic stress in higherplants: a review. Environmental Pollution, 147, 422-428.

[41]Liang Y C, Zhang W Q, Chen J, Ding R. 2005. Effect ofsilicon on H+-ATPase and H+-PPase activity, fatty acidcomposition and fluidity of tonoplast vesicles from rootsof salt-stressed barley (Hordeum vulgare L.).Environmental and Experimental Botany, 53, 29-37.

[42]Lux A, Luxova M, Hattori T, Inanaga S, Sugimoto Y. 2002.Silicification in sorghum (Sorghum bicolor) cultivar withdifferent drought tolerance. Physiologia Plantarum,115, 87-92.

[43]Müller-Moulé P, Golan T, Niyogi K K. 2004. Ascorbatedeficientmutants of Arabidopsis grow in high lightdespite chronic photooxidative stress. Plant Physiology,134, 1163-1172.

[44]Ma J F, Yamaji N. 2008. Functions and transport of siliconin plants. Cellular and Molecular Life Sciences, 65,3049-3057.

[45]Mali M, Aery N C. 2008. Influence of silicon on growth,relative water contents and uptake of silicon, calciumand potassium in wheat grown in nutrient solution.Journal of Plant Nutrition, 31, 1867-1876.

[46]McCree K J. 1986. Whole-plant carbon balance osmoticadjustment to drought and salinity stress. AustralianJournal of Plant Physiology, 13, 33-43.

[47]Medhat M T. 2002. Comparative study on growth, yieldand nutritive value for some forage plants grown underdifferent levels of salinity. Ph D thesis, Faculty ofScience, Botany Department, Cairo University, Egypt.

[48]Meloni D A, Oliva M A, Marinez C A, Cambraia J. 2003.Photosynthesis and ability of superoxide dismutase,peroxidase and glutathione reductase in cottonundersalt stress. Environmental and ExperimentalBotany, 49, 69-76.

[49]Metcalf L C, Schmitz AA, Pelka J R. 1966. Rapid preparationof methyl esters from lipid for gas chromatographyanalysis. Analytical Chemistry, 38, 514-515.

[50]Mittler R. 2002. Oxidative stress, antioxidants and stresstolerance. Trends Plant Science, 7, 405-410.

[51]Miyake C, Asada K. 1992. Thylakoid bound ascorbateperoxidase in spinach chloroplasts and photoreductionof its primary oxidation product, monodehydroascorbateradicals in the thylakoids. Plant and Cell Physiology,33, 541-553.

[52]Moussa H R. 2006. Influence of exogenous application ofsilicon on physiological response of salt-stressed maize(Zea mays L.). International Journal of Agricultureand Biology, 8, 293-297.

[53]Nakano Y, Asada K. 1987. Purification of ascorbateperoxidase in spinach chloroplast: in inactivation inascorbate-depleted medium and reactivation bymonodehydroascorbate radical. Plant Cell Physiology,28, 131-140.

[54]Nieman R H. 1962. Some effects of sodium chloride ongrowth, photosynthesis and respiration of twelve cropplants. Botanical Gazette, 123, 279-285.

[55]Pal M, Singh D K, Rao L S, Singh K P. 2004. Photosyntheticcharacteristics and activity of antioxidant enzymes insalinity tolerant and sensitive rice cultivars. IndianJournal of Plant Physiology, 9, 407-412.

[56]Qian Q Q, Zai W S, Zhu Z J, Yu J Q. 2006. Effects ofexogenous silicon on active oxygen scavengingsystems in chloroplasts of cucumber (Cucumis sativusL.) seedlings under salt stress. Journal of PlantPhysiology and Molecular Biology, 32, 107-112.

[57]Quereghi Z, Zid E, Ayadi A. 1991. Sensivity to NaCl andexclusion of Na+ in canola. Agriculture Mediterranean,121, 4-110.

[58]Rafi M M, Epstein E. 1999. Silicon absorption by wheat.Plant Soil, 211, 223-230.

[59]Rao K R, Gnaham A. 1990. Inhibition of nitrate and nitratereductase activity by salinity stress in sorghum vulgare.Phytochemistry, 29, 1047-1049.

[60]Raza S H, Athar H R, Ashraf M. 2006. Influence ofexogenous ly appli ed glycinebeta ine on thephotosynthetic capacity of two differently adapted wheatcultivars under salt stress. Pakistan Journal of Botany,38, 341-351.

[61]Romero-Aranda M R, Jurado O, Cuartero J. 2006. Siliconalleviates the deleterious salt effect on tomato plantgrowth by improving plant water status. Journal ofPlant Physiology, 163, 847-855.

[62]Savant N K, Snyder G H, Datnoff L E. 1997. Siliconmanagement and sustainable rice production. In: SparksD L, ed., Advances in Agronomy. vol. 58. AcademicPress, San Diego, CA. pp. 151-199.

[63]Savant N K, Korndorfer G H, DatnoffL E, Snydre G H. 1999.Silicon nutrition and sugarcane production: a review.Journal of Plant Nutrition, 22, 1853-1903.

[64]Schwarz M, Gale J. 1981. Maintenance respiration andcarbon balance of plants at low levels of sodium chloridesalinity. Journal of Experimental Botany, 32, 933-941.

[65]Shannon M C. 1984. Breeding, selection, and the geneticsof salt tolerance. In: Staples R C, ed., Salinity Tolerancein Plants: Strategies for Crop Improvement. Wiley, NewYork, NY. pp. 231-254.

[66]Smaoui A, Cherift A. 2000. Changes in molecular species oftriacylglycerols in developing cottonseeds under saltstress. Biochemical Society Transactions, 28, 902-905.

[67]Smirnoff N. 2000a. Ascorbate biosynthesis and function inphoto protection. Biochemical Society Transactions,15, 1455-1465.

[68]Smirnoff N. 2000b.Ascorbic acid: metabolism and functionsof a multifaceted molecule. Current Opinion in PlantBiology, 3, 229-235.

[69]Smirnoff N. 2005. Ascorbate, tocopherol and carotenoids:metabolism, pathway engineering and functions. In:Smirnoff N, ed., Antioxidants and Reactive OxygenSpecies in Plants. Blackwell Publishing, Oxford, UK.pp. 53-86.

[70]Strogonov B P, Kabanov V V, Pakova M M .1970. Featureof protein and nucleic acid metabolism during formativechanges in plant under salinization conditions. SovietPlant Physiology, 17, 394-397.

[71]Tuna A L, Kaya C, Higgs C D, Murillo-Amador B, AydemirS, Girgin A R. 2008. Silicon improves salinity tolerancein wheat plants. Environmental and ExperimentalBotany, 62, 10-16.

[72]Wright G C, Smith C J, Woodroofe M R. 1988. The effect ofirrigation and nitrogen fertilizer on rapeseed (Brassicanapus) production in south-eastern Australia: I. growthand seed yield. Irrigation Science, 9, 1-13.

[73]Zhu Z J, Wei G Q, Li T, Qian Q Q, Yu J Q. 2004. Siliconalleviates salt stress and increases antioxidant enzymesactivity in leaves of salt-stressed cucumber (Curcumassativa L.). Plant Science, 167, 527-533.