Preparation, Characterization and Nematicidal Activity of Lansiumamide B Nano-Capsules

doi:10.1016/S1671-2927(00)8641

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (7): 1151-1158.DOI: 10.1016/S1671-2927(00)8641

Preparation, Characterization and Nematicidal Activity of Lansiumamide B Nano-Capsules

YIN Yan-hua, GUO Qing-ming, HAN Yun, WANG Ling-jing, WAN Shu-qing

Key Laboratory of Natural Pesticide & Chemical-Biology of Education Ministry/South China Agricultural University, Guangzhou 510642,P.R.China

收稿日期:2011-09-07 出版日期:2012-07-01 发布日期:2012-07-27
通讯作者: WAN Shu-qing, Tel: +86-20-85285813, E-mail: wanshuqing1953@yahoo.com.cn
作者简介:YIN Yan-hua, Mobile: 13544495468, E-mail: yinyanhua1986000@163.com
基金资助:
This work was supported by Guangzhou Science and Technology Plan Projects, Guangzhou, China (10C62101593).

Preparation, Characterization and Nematicidal Activity of Lansiumamide B Nano-Capsules

YIN Yan-hua, GUO Qing-ming, HAN Yun, WANG Ling-jing, WAN Shu-qing

Key Laboratory of Natural Pesticide & Chemical-Biology of Education Ministry/South China Agricultural University, Guangzhou 510642,P.R.China

Received:2011-09-07 Online:2012-07-01 Published:2012-07-27
Contact: WAN Shu-qing, Tel: +86-20-85285813, E-mail: wanshuqing1953@yahoo.com.cn
About author:YIN Yan-hua, Mobile: 13544495468, E-mail: yinyanhua1986000@163.com
Supported by:
This work was supported by Guangzhou Science and Technology Plan Projects, Guangzhou, China (10C62101593).

摘要/Abstract

摘要： In this study, nano-capsules of lansiumamide B (NCLB) was prepared by the microemulsion polymerization method to improve the nematicidal efficacy of lansiumamide B (LB). An optimal formulation was gained by orthogonal experiment design based on the encapsulation efficiency (En, %) value. The optimized NCLB were spherical and uniform under transmission electron microscopy (TEM). The mean particle size, zeta potential and En were (38.50±0.64) nm, (-70.5±0.76) mV and (95.13±1.16)%, respectively. The release profile indicated that the accumulated release of LB in NCLB reached up to 82% within 96 h. Effects of NCLB against Bursaphelenehus xylophilus and J2 of Meloidogyne incognita were reported in this paper. The nematicidal activity of NCLB has been remarkably increased, with LC50 values of 2.1407 mg L-1 and 19.3608 mg L-1, respectively, at 24 h after treatment. The disease progression and the average number of root knots of Ipomoea aquatica were 1.50 and 7.25, respectively, in the treatment of NCLB, at concentration of 200 mg L-1, significantly lower than the treatment of LB and ethoprophos. Compared to control, the treatments of NCLB, LB and ethoprophos leaded the disease progression to drop 68.42, 36.84 and 26.32%, respectively, and caused the average number of root knots to fall 83.94, 78.03 and 63.66%. These results suggested that NCLB, as a novel nematicides formulation, performed more efficient and longer effective maintenance against plant parasitic nematodes.

关键词: lansiumamide B, nano-capsules, structure characterization, nematicidal activity

Abstract: In this study, nano-capsules of lansiumamide B (NCLB) was prepared by the microemulsion polymerization method to improve the nematicidal efficacy of lansiumamide B (LB). An optimal formulation was gained by orthogonal experiment design based on the encapsulation efficiency (En, %) value. The optimized NCLB were spherical and uniform under transmission electron microscopy (TEM). The mean particle size, zeta potential and En were (38.50±0.64) nm, (-70.5±0.76) mV and (95.13±1.16)%, respectively. The release profile indicated that the accumulated release of LB in NCLB reached up to 82% within 96 h. Effects of NCLB against Bursaphelenehus xylophilus and J2 of Meloidogyne incognita were reported in this paper. The nematicidal activity of NCLB has been remarkably increased, with LC50 values of 2.1407 mg L-1 and 19.3608 mg L-1, respectively, at 24 h after treatment. The disease progression and the average number of root knots of Ipomoea aquatica were 1.50 and 7.25, respectively, in the treatment of NCLB, at concentration of 200 mg L-1, significantly lower than the treatment of LB and ethoprophos. Compared to control, the treatments of NCLB, LB and ethoprophos leaded the disease progression to drop 68.42, 36.84 and 26.32%, respectively, and caused the average number of root knots to fall 83.94, 78.03 and 63.66%. These results suggested that NCLB, as a novel nematicides formulation, performed more efficient and longer effective maintenance against plant parasitic nematodes.

Key words: lansiumamide B, nano-capsules, structure characterization, nematicidal activity

YIN Yan-hua, GUO Qing-ming, HAN Yun, WANG Ling-jing, WAN Shu-qing. Preparation, Characterization and Nematicidal Activity of Lansiumamide B Nano-Capsules[J]. Journal of Integrative Agriculture, 2012, 12(7): 1151-1158.

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Preparation, Characterization and Nematicidal Activity of Lansiumamide B Nano-Capsules

Preparation, Characterization and Nematicidal Activity of Lansiumamide B Nano-Capsules

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