Transcriptome response of wheat Norin 10 to long-term elevated CO2 under high yield field condition

doi:10.1016/S1671-2927(00)10702

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (9): 2142-2152.DOI: 10.1016/S1671-2927(00)10702

收稿日期:2016-03-31 出版日期:2016-09-02 发布日期:2016-09-02

Transcriptome response of wheat Norin 10 to long-term elevated CO₂ under high yield field condition

LIN Yue-bing^1*, SHEN Cheng-guo^2*, LIN Er-da¹, HAO Xing-yu³, HAN Xue¹

¹ Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/Key Laboratory of Agricultural Environment, Ministry of Agriculture, Beijing 100081, P.R.China
² Bioinformatics Unit, Source BioScience, Nottingham NG8 6PX, UK
³ College of Agronomy, Shanxi Agricultural University, Taigu 030801, P.R.China

Received:2016-03-31 Online:2016-09-02 Published:2016-09-02
Contact: LIN Er-da, E-mail: linerda@caas.cn
About author:LIN Yue-bing, E-mail: paradelynn@163.com; SHEN Cheng-guo, E-mail: success.100081@gmail.com;
Supported by:
The authors thank financial supports from the National Basic Research Program of China (973 Program, 2012CB955904) and the Agricultural Science and Technology Innovation Program (ASTIP) of Chinese Academy of Agricultural Sciences.

摘要/Abstract

Abstract: The increasing atmospheric carbon dioxide concentration, caused by fossil fuel combustion and deforestation, plays an important role in plant growth and development. Wheat, as a major staple crop, adapts to climate change by tuning its inherent molecular mechanism, which is not well understood. The present study employed the RNA-Seq method to generate transcriptome profiles of the wheat Norin 10 in response to elevated CO₂ in comparison with ambient CO₂. The 10 895 787 high-quality clean reads of Norin 10 were assembled de novo using Trinity (without a reference genome) resulting in a total of 18 206 candidate transcripts with significant BLAST matches. GO enrichment analysis of Norin 10 at different CO₂concentrations showed that some functional genes related to plastids, precursor metabolites, and energy, thylakoid and photosynthesis were apparently enriched at elevated CO₂ (550 μmol mol^–1) in contrast to that at ambient CO₂ (400 μmol mol–1); these findings were further confirmed by RT-PCR analysis. The findings demonstrated the specific effects of elevated CO₂ during long-term period in free air CO2 enrichment (FACE) on transcriptome response of the high yielding wheat variety, Norin 10, which has a large spike.

Key words: Norin 10 , transcriptome response , wheat , long term elevated carbon dioxide , GO enrichment

LIN Yue-bing, SHEN Cheng-guo, LIN Er-da, HAO Xing-yu, HAN Xue. [J]. Journal of Integrative Agriculture, 2016, 15(9): 2142-2152.

LIN Yue-bing, SHEN Cheng-guo, LIN Er-da, HAO Xing-yu, HAN Xue. Transcriptome response of wheat Norin 10 to long-term elevated CO₂ under high yield field condition[J]. Journal of Integrative Agriculture, 2016, 15(9): 2142-2152.

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Transcriptome response of wheat Norin 10 to long-term elevated CO₂ under high yield field condition

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