IGF1-PI3K-Akt信号通路相关基因在黄羽肉鸡肌肉和肝脏中的表达

束婧婷,姬改革,单艳菊,章明,巨晓军,刘一帆,屠云洁,盛中伟,唐燕飞,蒋华莲,邹剑敏

Expression Analysis of IGF1-PI3K-Akt-Dependent Pathway Genes in Skeletal Muscle and Liver Tissue of Yellow Feather Broilers

SHU JingTing,JI GaiGe,SHAN YanJu,ZHANG Ming,JU XiaoJun,LIU YiFan,TU YunJie,SHENG ZhongWei,TANG YanFei,JIANG HuaLian,ZOU JianMin

表1 基因引物序列

Table 1 Gene-specific primers for quantitative RT-PCR

基因名称 Gene name 引物序列 Primer sequence PCR条件 PCR conditions (℃) IGF1 5′ primer: AGCTGGTTGATGCTCTTCAGTTCGT 60 3′ primer: TCCTCAGGTCACAACTCTGGAAGC IGF1R 5′ primer: TTCAGGAACCAAAGGGCGA 55 3′ primer: TGTAATCTGGAGGGCGATACC IRS1 5′ primer: CTACCGCCTGTGCCTGACTAAC 60 3′ primer: CGTCCCACCTCGATGAAGAAG HSP70 5′ primer: TCTGCTCCTGTTGGATGTC 60 3′ primer: TGGGAATGGTGGTGTTACG