琅琊鸡及其配套系蛋壳质量、钙代谢生化指标和钙结合蛋白CaBP-D28k mRNA表达的比较

张宁波,韩照清,金太花,庄桂玉,李炯奎,郑全胜,李永洙

Comparison Analysis on Eggshell Quality, Biochemical Index of Calcium Metabolism and Calcium Binding Protein CaBP-D28k mRNA Expression Between Langya Chicken and Its Synthetic Lines

ZHANG NingBo,HAN ZhaoQing,JIN TaiHua,ZHUANG GuiYu,LI JiongKui,ZHENG QuanSheng,LI YongZhu

表2 Real-Time PCR引物序列

Table 2 Primer sequences for RT-PCR

基因 Gene 基因编号 GenBank ID 引物序列 Sequence of primers (sense/antisense) (5′-3′) 产物大小 Length (bp) 钙结合蛋白 CaBP-D28k NM_205513 5′-TTAAATCTGCGTTGCTTCCATACA-3′ -3′ 298 5′-GGCCCATCCTGCACTCCATAAC-3′ -3′ β肌动蛋白 β-actin NM_205518 5′-CCCAAGGCCAACCGTGAGAAGAGT-3′ -3′ 337 5′-CGAAGTCCAGGGCCACGTAGCAGAG-3′ -3′