葡萄钾离子转运基因VviHKT1;7在盐胁迫下的功能鉴定

刘闯,高振,姚玉新,杜远鹏

Functional Identification of Grape Potassium Ion Transporter VviHKT1;7 Under Salt Stress

LIU Chuang,GAO Zhen,YAO YuXin,DU YuanPeng

表1 基因扩增及定量所用的引物

Table 1 Primers used for gene amplification and quantification

基因名称 Gene name 引物序列 Primer sequence 用途 Purpose VvActin F: 5′-TCTCAACCCAAAGGCTAATC-3′ R: 5′-GCATAGAGGGAAAGAACAGC-3′ qRT-PCR VviHKT1;1 F: 5′-ATAGATGGTGGTGAAAAGGCT-3′ R: 5′-GTTGTGATAACAGTAGATGCTCC-3′ qRT-PCR VviHKT1;2 F:5′-TGAGAGAGACAAGATGAGGGAAG-3′ R:5′-ACAAATCCATACCAGGCGTC-3′ qRT-PCR VviHKT1;3 F:5′-GAGCATCGCCCTGGAAGTC-3′ R:5′-TGCCGAGAACAGTGATACCC-3′ qRT-PCR VviHKT1;6 F:5′-AAATGGAGTGACGAGGGGA-3′ R:5′-TAGCCTTCAGACTTTGCCTC-3′ qRT-PCR VviHKT1;7 F:5′-CCATCTTCATCATCCTTATCTGC-3′ R:5′-CTCTACCCAACTATTAATCCCGG-3′ qRT-PCR VviHKT1;7 F:5′-ATGATGAACTCTGCTAGTTTCACC-3′ R:5′-AAAGCCCCTGATTACTTCTATCAC-3′ 扩增基因 Amplified gene VviHKT1;8 F:5′-CTTGTAAGTTGTAACCGAGGCA-3′ R:5′-CATGCATTGTGGATAGCTAAGG-3′ qRT-PCR