柑橘叶斑驳病毒的逆转录重组酶聚合酶扩增检测

段玉,许建建,马志敏,宾羽,周常勇,宋震

Detection of Citrus Leaf Blotch Virus by Reverse Transcription- Recombinase Polymerase Amplification (RT-RPA)

DUAN Yu,XU JianJian,MA ZhiMin,BIN Yu,ZHOU ChangYong,SONG Zhen

图5 CLBV RT-RPA检测体系的灵敏度 A：RT-PCR;B：RT-RPA;C：RT-qPCR。M：DNA分子质量标记DL2000 Marker;10-1—10-6：浓度为1.17×10-1—1.17×10-6 μg·μL-1 CLBV阳性核酸1.17×10-1 -1.17×10-6 μg·μL-1 CLBV nucleic acid standard

Fig. 5 Sensitivity of RT-RPA for CLBV detection