柑橘叶斑驳病毒的逆转录重组酶聚合酶扩增检测

段玉,许建建,马志敏,宾羽,周常勇,宋震

Detection of Citrus Leaf Blotch Virus by Reverse Transcription- Recombinase Polymerase Amplification (RT-RPA)

DUAN Yu,XU JianJian,MA ZhiMin,BIN Yu,ZHOU ChangYong,SONG Zhen

图1 CLBV RT-RPA引物的特异性检测 M：DNA分子质量标记DL2000 Marker;1、2、5、6、9、10：CLBV阳性核酸CLBV positive nucleic acid;3、7、11：健康对照CLBV negative control;4、8、12：ddH2O

Fig. 1 Specificity of three RT-RPA primer pairs for CLBV detection