柑橘叶斑驳病毒的逆转录重组酶聚合酶扩增检测
|
|
段玉,许建建,马志敏,宾羽,周常勇,宋震
|
Detection of Citrus Leaf Blotch Virus by Reverse Transcription- Recombinase Polymerase Amplification (RT-RPA)
|
|
DUAN Yu,XU JianJian,MA ZhiMin,BIN Yu,ZHOU ChangYong,SONG Zhen
|
|
表1 CLBV检测所用引物序列
|
Table 1 Primers sequences for the detection of CLBV
|
|
检测方法
Method | 引物名称
Primer | 引物序列
Primer sequence (5′-3′) | 引物序列位置
Primer sequence position (bp) | | RT-RPA | RPACF1 | TTAGCACTTTAACCTCAGACCCCTTACTTGG | 2460-2491 | | RPACR1 | GATAAAAGCCGCCTTCCTTCCTATGAACTGG | 2560-2590 | | RCLBV-F | ATGAACACTCACGGCGATGAAATTCCCACA | 2108-2137 | | RCLBV-R1 | TTCTGCAAGTGAAACATAGGATCCCCCATT | 2240-2269 | | RCLBV-R2 | GGATCCCCCATTAAATTCCATAAGCCAGTC | 2222-2251 | | RT-PCR | CLBV1F | AGCCATAGTTGAACCATTCCTC | 7159-7180 | | CLBV5R | GCAGATCATTCACCACATGC | 7564-7583 | | RT-qPCR | CLBVF | CAATTGCATGAACACTCACGG | 2101-2121 | | CLBVR | GGACCCCCCATTAAATTCCA | 2251-2231 |
|
|
|