禾谷镰孢核孔蛋白基因FgNup42的功能分析

张承启,廖露露,齐永霞,丁克坚,陈莉

Functional Analysis of the Nucleoporin Gene FgNup42 in Fusarium graminearium

ZHANG ChengQi,LIAO LuLu,QI YongXia,DING KeJian,CHEN Li

表1 本研究所用到的引物

Table 1 Primers used in this study

引物 Primer 序列 Sequence (5′-3′) Nup42-up-F GCGCTCTCAAGAGAGTCACCG Nup42-up-R CAAAATAGGCATTGATGTGTTGACCTCCCGCGGTAAGCTCTGGCTACCT Nup42-down-F CTCGTCCGAGGGCAAAGGAATAGAGTAGCATAGGTAGGTAGGTATCTAC Nup42-down-R TTGATTCCAGCCTCCAGCTG HPH-F GGAGGTCAACACATCAATGCCTATT HPH-R CTACTCTATTCCTTTGCCCT Nup42-nest-F CGAGGGCCATGTCATTCCTG Nup42-nest-R GAGTGAGATTCTAGGATCGT Nup42-ID-F GTATCTACTATGGCGATATTGC Nup42-ID-R GTATCCAACCAATCAGCGTGTC Nup42-GFP-F ACTCACTATAGGGCGAATTGGGTACTCAAATTGGTTGCGCTCTCAAGAGAGTCACCG Nup42-GFP-R CACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACAAAGTCCCACAATGTGCATTC ID-Nup42-GFP-F CCGCAAGCTAACAATCCATTC ID-GFP-R GTCAGCTTGCCGTAGGTGGCA Tri1-RT-F GATGTTCCTTCTCGACAGCGT Tri1-RT-R CACTGGTCGAAGATAGCTGG Tri3-RT-F TGTTACGATCAATGGCTTGG Tri3-RT-R TCCTCGTTGTAGTTTGCATCA Tri4-RT-F ACGTGTGGCTACTCAGGAGAA Tri4-RT-R TGGAATTGCCTTGGGGTA Tri6-RT-F AAATGCCCATTCCCTAGTTG Tri6-RT-R ATCTCGCATGTTATCCACCCT Tri7-RT-F TACCGTCGTCTTCAAAACCA Tri7-RT-R ACGCCAATGGTGTTCACAAA Tri8-RT-F ATATAACGGTACCCCCAGATG Tri8-RT-R TGTTTGTAGGACACTTCCGGT Tri14-RT-F AACTCCCGTTGTGATCAAGCA Tri14-RT-R AACAGTAATGTTGGCACCGT actin-RT-F CCACGTCACCACTTTCAACT actin-RT-R TGCTTGGAGATCCACATTTG