长江下游粳稻稻瘟病广谱抗性基因组合模式分析

吴云雨,肖宁,余玲,蔡跃,潘存红,李育红,张小祥,黄年生,季红娟,戴正元,李爱宏

Construction and Analysis of Broad-Spectrum Resistance Gene Combination Pattern for Japonica Rice in Lower Region of the Yangtze River, China

WU YunYu,XIAO Ning,YU Ling,CAI Yue,PAN CunHong,LI YuHong,ZHANG XiaoXiang,HUANG NianSheng,JI HongJuan,DAI ZhengYuan,LI AiHong

表1 用于检测目的基因的分子标记详细信息

Table 1 Detail information of molecular markers tightly linked to different resistant genes

基因 Gene 标记 Marker 引物序列 Primer sequence (5′-3′) 退火温度 Annealing temperature (℃) 预期片段大小 Expected size (bp) Pi2 Pi2-InDel F: GCAGCGGCTAGGGTTTATC 60 110 R: CACCCAGCAACTGATTTGTCA Pi9 M-Pi9 F: GCTGTGCTCCAAATGAGGAT 55 291 R: GCGATCTCACATCCTTTGCT Pi40 AP5659-5 F: CTCCTTCAGCTGCTCCTC 55 288 R: TGATGACTTCCAAACGGTAG Pigm Z4794 F: TGAATGTGAGAGGTTGACTGTGG 55 334 R: CACGCCACCCTTCAATGGAGACT Pizt AP22 F: GTGCATGAGTCCAGCTCAAA 58 143 R: GTGTACTCCCATGGCTGCTC Piz S29742 F: CAGTGAAACGAACGCTATG 55 454 R: AATAGGAAGGGTTGATGTTG Pi1 RM224 F: ATCGATCGATCTTCACGAGG 55 163 R: TGCTATAAAAGGCATTCGGG Pi54 Pi54-1 F: ATCGATCGATCTTCACGAGG 55 216 R: GCTTCAATCACTGCTAGACC Pi33 RM72 F: CCGGCGATAAAACAATGAG 55 240 R: GCATCGGTCCTAACTAAGGG