花后DA-6处理调控小麦种子活力的机理

许晨,王文静,曹珊,李如雪,张贝贝,孙爱清,张春庆

Mechanism of DA-6 Treatment Regulating Wheat Seed Vigor After Anthesis

XU Chen,WANG WenJing,CAO Shan,LI RuXue,ZHANG BeiBei,SUN AiQing,ZHANG ChunQing

表1 qRT-PCR引物

Table 1 Primers used for qRT-PCR

基因Gene 引物名称Primer name 序列Sequence (5′-3′) 肌动蛋白基因（内参）Actin gene (internal reference) Actin-F CGAAGCGACATACAATTCCATC Actin-R GAACCTCCACTGAGAACAACAT 蛋白磷酸酶基因Protein phosphatase gene TraesCS3B02G277900-F CTGGCAGGTGGTTGGAGGAAT TraesCS3B02G277900-R GCAATAGGCTCAGGACGGATCT 蔗糖磷酸合成酶基因Sucrose phosphate synthase gene TraesCS3A02G015500-F GTGCCAATGGTCTTCACAGGTC TraesCS3A02G015500-R TCAGCCTCAATCCGTCGCATTA 蛋白质二硫键异构酶基因Protein disulfide isomerase gene TraesCS4A02G214200-F CCCTGACAACCATCCTTACCTCT TraesCS4A02G214200-R CCTTGCCACTGAACTCCTCTACA hsp82 TraesCS2A02G033700-F GCTGCTCTCGCTCATCATCAAC TraesCS2A02G033700-R TGCTCTTGTCTGTCAGGCTCTC Ⅰ类hsp ClassⅠ hsp TraesCS4B02G211700-F ACAAGGAGCAGGAGGAGAAGAC TraesCS4B02G211700-R TGACGGTGAGCACGCTGTT hsp70 TraesCS1D02G284000-F CGGCATCCTGAACGTGTCTG TraesCS1D02G284000-R TCTTCTTGTGCTCCTCGTCCTC