捻转血矛线虫Hc-hrg-2拯救血红素缺陷型酵母生长表型

周静茹,吴飞,陈学秋,黄艳,时恒枝,杜爱芳,杨怡

Hc-hrg-2 of Haemonchus Contortus Rescues the Growth of Heme Deficient Yeast Strain

ZHOU JingRu,WU Fei,CHEN XueQiu,HUANG Yan,SHI HengZhi,DU AiFang,YANG Yi

图2 hem1敲除组件的构建 M1：DL250 DNA marker; M2：DL10000 DNA marker; 1-3：依次为上游同源臂、URA3、下游同源臂的PCR产物;4：敲除组件的两次重叠PCR的终产物

Fig. 2 The construction of hem1 knockout component M1: DL250 DNA marker; M2: DL10000 DNA marker; 1 - 3: PCR products of upstream homology sequence, URA3, and downstream homology sequence; 4: Final product of two overlapping PCRs of knockout components