捻转血矛线虫Hc-hrg-2拯救血红素缺陷型酵母生长表型

周静茹,吴飞,陈学秋,黄艳,时恒枝,杜爱芳,杨怡

Hc-hrg-2 of Haemonchus Contortus Rescues the Growth of Heme Deficient Yeast Strain

ZHOU JingRu,WU Fei,CHEN XueQiu,HUANG Yan,SHI HengZhi,DU AiFang,YANG Yi

表1 本试验所用的引物序列

Table 1 Primer sequences used in this study

引物名称 Primer name 上下游引物（5'-3'）Primer 5' FR-F CACGGTTTCCTTTGCCAATT 5' FR-R TATGCTATACGAAGTTATACTGAAAAAAAAACCTAAGTACTGTTATG 3' FR-F TACATTATACGAAGTTATAACAACCAATATATGCATGGGCTGA 3' FR-R CTAAAGAATGTCCAAATATCGCCGG URA3-F ATAACTTCGTATAGCATACATTATACGAAGTTATTTCAATTCATCATTTTTT URA3-R ATAACTTCGTATAATGTATGCTATACGAAGTTATTTAGTTTTGCTGGCCGCA A CATAGGAAAACGGTTAAAAGGCCCTGCTTCTACC B GCTATTATGGAGGAACCCTGTTCAAACCGG C ATGCAACGCTCCATTTTTGC D TTACTGCTTGATACCACTAGAAACCTC E GCTGCTGCATGTGTTGATGACGCTG F CTTGAACCCTAATGTTAGAGACCCC G GAGAAGATGCGGCCAGCAAAACTAA H CTAAAGAATGTCCAAATATCGCCGGC I TCTGTGCTCCTTCCTTCGTTCTTCC J GAGAAGATGCGGCCAGCAAAACTAA Hc-hrg-2-F aattcaaccctcactaaagg ATGATTCTCTTGGTTTCTGTTGCTG Hc-hrg-2-R tcatccttgtaatccatcgaTTCTTCAGCAAACTCTTTTCCAAAAACCGTA Ce-hrg-4-F aattcaaccctcactaaaggATGACTGCTGAAAATCGAGGATTCT Ce-hrg-4-R tcatccttgtaatccatcgaACTTTTAATGACTTCAACATCGTCATC pESC-F TGTCAACAACGTATCTACCA pESC-R GGCTCTTTACATTTCCACAA