姜黄素通过Nrf2信号通路对H2O2诱导奶牛乳腺上皮细胞氧化应激的缓解

姜春晖,孙旭东,唐燕,罗胜缤,徐闯,陈媛媛

Curcumin Alleviates H2O2-Induced Oxidative Stress in Bovine Mammary Epithelial Cells Via the Nrf2 Signaling Pathway

JIANG ChunHui,SUN XuDong,TANG Yan,LUO ShengBin,XU Chuang,CHEN YuanYuan

表1 Real-time PCR分析所用引物

Table 1 Gene-specific primers of Real-time PCR

基因 Gene 引物序列 Primer sequences (5′-3′) 产物大小 Length (bp) Nrf2 F GCCCTCACTGGATAAAGAA 202 R CATGCCGTTGCTGGTAC NQO1 F AACCAACAGACCAGCCAATC 154 R CACAGTGACCTCCCATCCTT HO-1 F CAAGGAGAACCCCGTCTACA 225 R CCAGACAGGTCTCCCAGGTA β-Actin F GCCCTGAGGCTCTCTTCCA 101 R GCGGATGTCGACGTCACA GAPDH F TCTTCACTACCATGGAGAAGG 197 R TCATGGATGACCTTGGCCAG