nuoB对莓实假单胞菌生理特性及在冷鲜鸡肉中致腐能力的影响

王光宇,李晴,唐文倩,王虎虎,徐幸莲,邱伟芬

Effects of nuoB on Physiological Properties of Pseudomonas fragi and Its Spoilage Potential in Chilled Chicken

WANG GuangYu,LI Qing,TANG WenQian,WANG HuHu,XU XingLian,QIU WeiFen

表1 本研究中所用的引物

Table 1 Primers used in this study

引物 Primer 序列（5′-3′） Sequence (5′-3′) nuoB-F ATATCTAGACCTGCATAGACATTGATGGAGGTTCTAC AAC nuoB-R ATATCTAGACTTGATCATCACGTCGCTGTTACGTTC Kpn-Kn-F ATATATATGGTACCGGAATAGGGAACTTCAAGATCCC CTC Kpn-Kn-R ATATATATGGTACCAGAGCGCTTTTGAAGCTGG nuoB-outF GAGGCCTGCGACATAGCGACACAAC nuoB-outR CAGACTTCGCGCTCGTACCAGTTGG 下划线代表限制性内切酶酶切位点 Underline sequences are cleavage sites of restriction enzyme