利用荧光SSR分子标记评估中国栗属植物遗传多样性

聂兴华,郑瑞杰,赵永廉,曹庆芹,秦岭,邢宇

Genetic Diversity Evaluation of Castanea in China Based on Fluorescently Labeled SSR

NIE XingHua,ZHENG RuiJie,ZHAO YongLian,CAO QingQin,QIN Ling,XING Yu

表2 12对高质量SSR标记的信息表

Table 2 The information of 12 SSR markers

序号 Number 标记名 Marker ID DNA序列号 GenBank ID 上游引物 Forward primer (5′-3′) 下游引物 Reverse primer (3′-5′) 观察基序 Repeat motif 1 CmSI0396 290474606 AACTCCCACCACTCACATCC TTTCGGACCATCCAGAACTC CACACC 2 CmSI0561 290474702 CGTATAGGGTGGAAACGGAA GGACAAGCAAATCACGGAAT TCG 3 CmSI0614 290476556 TTGTGGTGAAGCTGACATCG GGGTACTACCACAACATGCAG GTT 4 CmSI0658 290474781 AAAACGGTTTGTGGTGAAGC GCCAACCAGTCAAGGGTACT GTT 5 CmSI0702 290474818 GAAACACACCAGAGAGATGCAG TTTTATACAGAGACATACTATCCTACACAG TC 6 CmSI0742 290474850 GACGCTCCTCAGCTTTTGAC TGCCGGTCAATTCTTCTTCT AG 7 CmSI0800 290474899 TTATGGCAACCCTCCTGTTT CTGAAATGATCGATGCTGCT TC 8 CmSI0853 290474950 GGAGGAGGAGGAGCTCATTG CCTTGGAGAGCTGCCAGTAG TCT 9 CmSI0871 290474967 AGGGGGTGGAAGAACCTATG AGATTGCAAGTGGGGAATTG TCT 10 CmSI0883 290476060 CAGCATCAGCACTCGTTCA GGGATTGAGAGGATGAAGCA AGC 11 CmSI0922 290475011 AATCTGAACCCCTCCGATCT ACCAACAACATGTGCCAAAA TTG 12 CmSI0930 290475019 CCATTTAGCATGCATAGTCATACC GCAAGGATGTAGGTCGAATCA ATAC