基于小麦SNP芯片对簇毛麦6V#2和6V#4染色体及其与小麦6A、6D染色体的多态性分析

许志英,王佰翠,马晓兰,贾子苗,叶兴国,林志珊,胡汉桥

Polymorphism Analysis Among Chromosomes of Dasypyrum villosum 6V#2 and 6V#4 and Wheat 6A and 6D Based on Wheat SNP Chip

XU ZhiYing,WANG BaiCui,MA XiaoLan,JIA ZiMiao,YE XingGuo,LIN ZhiShan,HU HanQiao

表2 PCR分子标记引物序列信息

Table 2 Information on the molecular markers used in PCR amplification

引物名称 Primer name 正向引物 Forward primer (5'-3') 反向引物 Reverse primer (5'-3') 检测类型 Types detected 参考文献 References 6VS-06 GACAGGCAGCTATGAGGC AATCGTCGTTTGGAGTGG 6V#4S [20] 6VS-09 GTAAGAACAAGAGGCTAAACAG CCAGATGACGGTTATTACATAG 6V#4S [20] 6VS-10 GCCATAAGTGACGCTGAT GCATCCTGTGAAGTTGTTG 6V#4S [20] 6VS-12 TGTTGCCTCTCCTCATCA ATTGCTGTCCGCTCATAC 6V#2S 6V#4S [20] 6VS-15 AGGACCATACATTCACAGAG TTCCATGAGCAGATTAGCA 6V#2S 6V#4S [20] 6VS-18 AGCCAGTAAGATTCCGTATG TCTAACCTTCCTCACAACAC 6V#4S [20] MBH1 GCCATTATAGTCAAGAGTGCACTAGCTGT AGCTCCTCTCGTTCTCCAATGCT 6V#2S 6V#4S 6AS;6DS [10] P259-1 CGTGATTCAGGAAATGCGATAC TTGCGCCGCCATGTTAG 6V#4S [8] P461-5a GCGTCATCCGCGCCCGTCAGGT GAGTGCTAATGATAGATGTG 6V#2S 6V#4S [8] N-P5 GCGACCTGTTAGAATGCTATTACGATTAC ATGCTACTCTACCGATGCTTTGAACC 6AS 6DS [12] 6V-1 CCGTGCGACAGAACAGAAGTGA GCAATCAGCCACATACAGGTCATC 6VL [20]