基于小麦SNP芯片对簇毛麦6V#2和6V#4染色体及其与小麦6A、6D染色体的多态性分析

许志英,王佰翠,马晓兰,贾子苗,叶兴国,林志珊,胡汉桥

Polymorphism Analysis Among Chromosomes of Dasypyrum villosum 6V#2 and 6V#4 and Wheat 6A and 6D Based on Wheat SNP Chip

XU ZhiYing,WANG BaiCui,MA XiaoLan,JIA ZiMiao,YE XingGuo,LIN ZhiShan,HU HanQiao

表1 PCR 扩增反应体系

Table 1 PCR amplification reaction system

组分 Component 用量 Dosage (μL) 2×Taq master mix 7.5 dd H2O 5.9 正向引物 Forward primer (10 μmol·L-1) 0.3 反向引物 Reverse primer (10 μmol·L-1) 0.3 DNA (100 ng·μL-1) 1.0 总计Total 15.0