不同施氮水平下灌浆期高温对水稻贮藏蛋白积累及其合成代谢影响

韩展誉,吴春艳,许艳秋,黄福灯,熊义勤,管弦悦,周庐建,潘刚,程方民

Effects of High-Temperature at Filling Stage on Grain Storage Protein Accumulation and Its Biosynthesis Metabolism for Rice Plants Under Different Nitrogen Application Levels

HAN ZhanYu,WU ChunYan,XU YanQiu,HUANG FuDeng,XIONG YiQin,GUAN XianYue,ZHOU LuJian,PAN Gang,CHENG FangMin

表1 荧光定量PCR引物序列

Table 1 The primer sequences for quantitative PCR

基因 Gene name 登录号 Accession No. 上游引物 Forward primer (5′-3′) 下游引物 Reverse primer (5′-3′) GluA1 KC202291 AAGACAGTGTTCAACGGCGA TTGGGAGAGCACGGAAGATG GluA2 KC202292 ATGATGGTGAAGTGCCGGTT CTCCTCAACTTCACGCCTGT GluA3 KC202293 ACAACCCAAACCTCGCAGAT CGGGCACGACCTTGAGTAAT GluB1 X54314 GCGTATCGCATCTCAAGGGA GGTCTCGCTTTCGGACTCAT GluB4 AK242284 GTCAATTGCTGATCATTCCA CTGCAAGGTGGCTCACCA GluB5 AY196923 GAGCGTGAAGGATGCCAAT GGTAAGGCGCGGAATACTGA Pro13 X14392 AGACTCAAGCTCAAGCCCA CACCAACAGTGGCAATGCTC Pro14 D11385 GCAATATCAGGTGCAGTCG ACTGCTGCCTTACGAACTCA Pro17 AB016505 GGTGAGTTCGTAAGGCAGCA GTGCGATCATCCTGAGCTGT Actin-1 X16280 CAGCACATTCCAGCAGATGT TAGGCCGGTTGAAAACTTTG