基于PK15细胞的猪圆环病毒2型全悬浮培养工艺
王嘉琪1(
),董育红1,姜菊玲1,钱建宁1,魏文涛1,宋国亮2,焦金波2,关新新2,姬郭彪2,张业炘1
),董育红1,姜菊玲1,钱建宁1,魏文涛1,宋国亮2,焦金波2,关新新2,姬郭彪2,张业炘1
Based on PK15 Cell Line for PCV2 Fully Suspension Culture Process
JiaQi WANG1(),YuHong DONG1,JuLing JIANG1,JianNing QIAN1,WenTao WEI1,GuoLiang SONG2,JinBo JIAO2,XinXin GUAN2,GuoBiao JI2,YeXin ZHANG1
),YuHong DONG1,JuLing JIANG1,JianNing QIAN1,WenTao WEI1,GuoLiang SONG2,JinBo JIAO2,XinXin GUAN2,GuoBiao JI2,YeXin ZHANG1
图2. PK15细胞感染PCV2后TCID50
A.PK15、PK15-2F11、PK15-1E5、PK15-1C8四株细胞分别感染PCV2后TCID50。B.PK15-2F11、PK15-1E5、PK15-1C8三株细胞分别对应感染图A收获PCV2病毒。其中,C1为使用接毒培养基A接毒实验组,C2为使用接毒培养基B接毒实验组。
Fig. 2. TCID50 after PCV2 infection in PK15 cells
A. TCID50 of PK15, PK15-2F11, PK15-1E5, PK15-1C8 cells infected with PCV2. B. PK15-2F11, PK15-1E5, and PK15-1C8 were infected with PCV2 virus harvest from Figure A. Among them, C1 is the experimental group infected with inoculation medium A, and C2 is the experimental group infected with inoculation medium B.
