基于PK15细胞的猪圆环病毒2型全悬浮培养工艺
王嘉琪1(
),董育红1,姜菊玲1,钱建宁1,魏文涛1,宋国亮2,焦金波2,关新新2,姬郭彪2,张业炘1
),董育红1,姜菊玲1,钱建宁1,魏文涛1,宋国亮2,焦金波2,关新新2,姬郭彪2,张业炘1
Based on PK15 Cell Line for PCV2 Fully Suspension Culture Process
JiaQi WANG1(),YuHong DONG1,JuLing JIANG1,JianNing QIAN1,WenTao WEI1,GuoLiang SONG2,JinBo JIAO2,XinXin GUAN2,GuoBiao JI2,YeXin ZHANG1
),YuHong DONG1,JuLing JIANG1,JianNing QIAN1,WenTao WEI1,GuoLiang SONG2,JinBo JIAO2,XinXin GUAN2,GuoBiao JI2,YeXin ZHANG1
图1. 4株单克隆细胞驯化悬浮生长曲线
A.PK15细胞驯化生长曲线。B.PK15-2F11细胞驯化生长曲线。C.PK15-1E5细胞驯化生长曲线。D. PK15-1C8细胞驯化生长曲线。其中,实线为细胞生长密度曲线,虚线为细胞活率曲线。浅色线为3种单克隆和PK15贴壁细胞生长曲线,深色线条为3种单克隆和PK15细胞驯化悬浮细胞生长曲线。
Fig. 1. Adaption suspension growth curves of four monoclonal cells
A. PK15 cells adapted growth curve. B. Acclimation growth curve of PK15-2F11 cells. C. Acclimation growth curve of PK15-1E5 cells. D. Acclimation growth curve of PK15-1C8 cells. Among them, the solid line is the cell growth density curve, and the dashed line is the cell viability curve. The light-colored line is the growth curve of 3 kinds of monoclonal and PK15 adherent cells, and the dark line is the growth curve of the acclimated suspension cells of 3 kinds of monoclonal and PK15 cells.
