抗寒无核葡萄杂种胚挽救及分子标记辅助选择

朱佩佩,罗燚佳,向雯,张明磊,张剑侠

Rescue and Molecular Marker Assisted-Selection of the Cold-Resistant Seedless Grape Hybrid Embryo

PeiPei ZHU,YiJia LUO,Wen XIANG,MingLei ZHANG,JianXia ZHANG

表2 胚发育培养基MM3与ER对胚挽救成苗的影响

Table 2 Effects of embryo development media MM3 and ER on embryo rescue

杂交组合 Crossing combination 培养基类型 Types of medium 胚珠数 No.of ovules cultured 剥胚数 No. of embryos developed 萌发数 No. of embryos germinated 成苗数 No. of Normal seedings 发育率 Proportion of Embryos developed (%) 萌发率 Proportion of Embryos germinated (%) 成苗率 Proportion of seedings (%) Ruby Seedless × Jupiter MM3 525 72 56 56 13.71a 10.67a 10.67a Ruby Seedless × Jupiter ER 508 58 35 35 11.42b 6.89b 6.89b 秦红2号×00-1-5 Qinhong No.2 × 00-1-5 MM3 405 69 38 30 17.04a 9.38b 7.41a 秦红2号×00-1-5 Qinhong No.2 × 00-1-5 ER 386 50 39 23 12.95b 10.10a 5.96b