vvi-miR172s及其靶基因响应赤霉素调控葡萄果实发育的作用分析
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宣旭娴,盛子璐,解振强,黄雨晴,巩培杰,张川,郑婷,王晨,房经贵
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Function Analysis of vvi-miR172s and Their Target Genes Response to Gibberellin Regulation of Grape Berry Development
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XuXian XUAN,ZiLu SHENG,ZhenQiang XIE,YuQing HUANG,PeiJie GONG,Chuan ZHANG,Ting ZHENG,Chen WANG,JingGui FANG
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表2 葡萄vvi-miR172s及靶基因信息
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Table 2 Information of vvi-miR172s and target genes in grapevine
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| miRNAs | MiRNA成熟
体长度
Length of miRNA | 靶基因编号
Target_Acc. | 靶基因长度
Length of target | 靶区
Target regions | 靶区起始、
终止位点
Target start and stop sites | 错配率
Expectation | 允许不匹配
的最大能量
UPE | 切割方式
Interaction mode | | vvi-miR172a/b | 21 | VIT_07s0031g00220(VvAP2) | 2565 | CDS:1870-1890 | C、C | 2.5 | 15.05 | 裂解Cleavage | | 21 | VIT_08s0040g03180(VvRAP2-1) | 1730 | CDS:1331-1351 | C、T | 2.5 | 11.489 | 裂解Cleavage | | 21 | VIT_13s0019g03550(VvRAP2-2) | 2217 | CDS:1566-1586 | C、C | 2.5 | 17.172 | 裂解Cleavage | | 21 | VIT_06s0004g03590(VvRAP2-3) | 2347 | CDS:1806-1826 | C、T | 2.5 | 14.04 | 裂解Cleavage | | vvi-miR172c | 21 | VIT_07s0031g00220(VvAP2) | 2565 | CDS:1870-1890 | C、C | 0.5 | 15.05 | 裂解Cleavage | | 21 | VIT_08s0040g03180(VvRAP2-1) | 1730 | CDS:1331-1351 | C、T | 1.0 | 11.489 | 裂解Cleavage | | 21 | VIT_13s0019g03550(VvRAP2-2) | 2217 | CDS:1566-1586 | C、C | 0.5 | 17.172 | 裂解Cleavage | | 21 | VIT_06s0004g03590(VvRAP2-3) | 2347 | CDS:1806-1826 | C、T | 1.0 | 14.04 | 裂解Cleavage | | vvi-miR172d | 23 | VIT_07s0031g00220(VvAP2) | 2565 | CDS:1870-1892 | C、A | 2.0 | 15.552 | 裂解Cleavage | | 23 | VIT_08s0040g03180(VvRAP2-1) | 1730 | CDS:1331-1353 | C、G | 1.5 | 9.197 | 裂解Cleavage | | 23 | VIT_13s0019g03550(VvRAP2-2) | 2217 | CSS:1566-1588 | G、A | 2.5 | 22.9 | 裂解Cleavage | | 23 | VIT_06s0004g03590(VvRAP2-3) | 2347 | CDS:1806-1828 | C、A | 0.5 | 14.075 | 裂解Cleavage |
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