vvi-miR172s及其靶基因响应赤霉素调控葡萄果实发育的作用分析

宣旭娴,盛子璐,解振强,黄雨晴,巩培杰,张川,郑婷,王晨,房经贵

Function Analysis of vvi-miR172s and Their Target Genes Response to Gibberellin Regulation of Grape Berry Development

XuXian XUAN,ZiLu SHENG,ZhenQiang XIE,YuQing HUANG,PeiJie GONG,Chuan ZHANG,Ting ZHENG,Chen WANG,JingGui FANG

表1 引物序列及用途

Table 1 Sequence and use of primers

引物名称 Primer name 引物序列 Primer sequence 引物用途 Primer use vvi-miR172a-F AAGCTTTCGTAGTTGTCTACAAAATGATCTCT vvi-miR172a前体序列的扩增 Amplification of vvi-miR172a precursor vvi-miR172a-R GGATCCCATCGCTTTACATTTTAGCCACT vvi-miR172b-F AAGCTTCGGCATCTTTCTCCTCATACC vvi-miR172b前体序列的扩增 Amplification of vvi-miR172b precursor vvi-miR172b-R GGATCCGGGTTGAAGACGTACATAAATCTCA vvi-miR172c-F AAGCTTTTTGAAGCGTTCTGCTGTC vvi-miR172c前体序列的扩增 Amplification of vvi-miR172c precursor vvi-miR172c-R GGATCCCAGATGAAACACTTCCAAGAGA vvi-miR172d-F AAGCTTGAAGCGAGATCCGTATCCTC vvi-miR172d前体序列的扩增 Amplification of vvi-miR172d precursor vvi-miR172d-F GGATCCATCTCAAATTACTGCATGATTTCAA Actin-F TACAATTCCATCATGAAGTGTGATG Actin内参引物 Actin internal reference primer Actin-R TTAGAAGCACTTCCTGTGAACAATG VvAP2 qRT-F CTCGCGCATATGATAGGGCT VvAP2定量RT-PCR引物 Real-time PCR of VvAP2 VvAP2 qRT-R ACTTTGTCGGCGAAGGACAT VvRAP2-1 qRT-F CAACACTGGGGGTTCATCCA VvRAP2-1定量RT-PCR引物 Real-time PCR of VvRAP2-1 VvRAP2-1 qRT-R TTTTGCCATGCCCAGTTTGG VvRAP2-2 qRT-F CTGACTACCGTGCAAGGGTT VvRAP2-2定量RT-PCR引物 Real-time PCR of VvRAP-22 VvRAP2-2 qRT-R TTTGATCACCCTGGCCTAGC VvRAP2-3 qRT-F GGGCTAGCAATGACATCGGA VvRAP2-3定量RT-PCR引物 Real-time PCR of VvRAP2-3 VvRAP2-3 qRT-R CATTTGCCACCCCCAGTTTG