马铃薯甲虫热激蛋白基因Ld-hsp70的克隆及温度胁迫下的表达特性
郑海霞1(
),高玉林2,张方梅2,3,杨超霞1,2,蒋健2,朱勋2,张云慧2(),李祥瑞2()
),高玉林2,张方梅2,3,杨超霞1,2,蒋健2,朱勋2,张云慧2(),李祥瑞2()
Cloning of Heat Shock Protein Gene Ld-hsp70 in Leptinotarsa decemlineata and Its Expression Characteristics under Temperature Stress
HaiXia ZHENG1(),YuLin GAO2,FangMei ZHANG2,3,ChaoXia YANG1,2,Jian JIANG2,Xun ZHU2,YunHui ZHANG2(),XiangRui LI2()
),YuLin GAO2,FangMei ZHANG2,3,ChaoXia YANG1,2,Jian JIANG2,Xun ZHU2,YunHui ZHANG2(),XiangRui LI2()
图2. 马铃薯甲虫5种Ld-hsp70的氨基酸序列比对
HSP70蛋白来源及GenBank登录号 Origin of HSP70 protein and their GenBank accession numbers：马铃薯甲虫Leptinotarsa decemlineata Ld-hsp70a（KC544268）;Ld-hsp70b（KC544269）;Ld-hsp70c（KC544270）;LdHSP70A（AF288978）;LdHSP70B（AF322911）。下划线：3段保守的HSP70家族签名序列Underlined: three conserved motifs of the HSP70 family;方框：胞质型标志性基序（EEVD）和内质网型HSPC末端标志性基序（KDEL）Box: the cytoplasmic HSP70 carboxyl terminal region (EEVD) and the endoplasmic reticulum HSP70 carboxyl terminal region (KDEL);箭头：C末端具有两个GGXP四肽序列Arrows: two GGXP quaternary peptide sequences at the C-terminal;黑色区域：高度保守的氨基酸Black blocks: highly conserved amino acids
Fig. 2. Alignment of five heat shock protein sequences of Ld-hsp70s in L. decemlineata
