马铃薯甲虫热激蛋白基因Ld-hsp70的克隆及温度胁迫下的表达特性
郑海霞1(
),高玉林2,张方梅2,3,杨超霞1,2,蒋健2,朱勋2,张云慧2(),李祥瑞2()
),高玉林2,张方梅2,3,杨超霞1,2,蒋健2,朱勋2,张云慧2(),李祥瑞2()
Cloning of Heat Shock Protein Gene Ld-hsp70 in Leptinotarsa decemlineata and Its Expression Characteristics under Temperature Stress
HaiXia ZHENG1(),YuLin GAO2,FangMei ZHANG2,3,ChaoXia YANG1,2,Jian JIANG2,Xun ZHU2,YunHui ZHANG2(),XiangRui LI2()
),YuLin GAO2,FangMei ZHANG2,3,ChaoXia YANG1,2,Jian JIANG2,Xun ZHU2,YunHui ZHANG2(),XiangRui LI2()
图1. Ld-hsp70 RT-PCR和3′ RACE产物
A：Ld-HSP70a筛选片段的PCR产物PCR product of Ld-HSP70a fragment;B：3′ RACE产物PCR product of 3′ RACE;C：全长ORF的PCR产物PCR product of full-length ORF;D：Ld-HSP70b筛选片段的PCR产物PCR product of Ld-HSP70b fragment;E：3′ RACE产物PCR product of 3′ RACE;F：全长ORF的PCR产物PCR product of full-length ORF;G：Ld-HSP70c筛选片段的PCR产物PCR product of Ld-HSP70c fragment;H：3′ RACE产物PCR product of 3′ RACE;I：全长ORF的PCR产物PCR product of full-length ORF;M1：100 bp ladder DNA maker;M2：BM2000 DNA maker;1—9（箭头Arrow）： PCR 产物 PCR products
Fig. 1. The RT-PCR and 3′ RACE products of Ld-hsp70s
