马铃薯Y病毒衣壳蛋白抗原表位分析及其快速ELISA检测方法的建立

梁雨欣,吴建祥,李小宇,张春雨,侯吉超,周雪平,王永志

Mapping of Epitopes and Establishment of Rapid DAS-ELISA for Potato Virus Y Coat Protein

YuXin LIANG,JianXiang WU,XiaoYu LI,ChunYu ZHANG,JiChao HOU,XuePing ZHOU,YongZhi WANG

表3 抗体最佳工作浓度确定

Table 3 Determination of working concentration of antibody

检测抗体浓度 Concentration of detection antibody (μg·mL-1) 捕获抗体浓度Concentration of capture antibody (μg·mL-1) 4 2 1 0.5 2 12.72±0.95* 7.56±0.93 5.10±0.53 4.05±0.13 1 7.44±0.77 4.42±0.07 2.82±0.12 2.46±0.01 0.5 5.17±0.36 3.31±0.04 2.34±0.19 2.03±0.07 0.25 2.87±0.25 2.14±0.01 1.81±0.18 1.59±0.15