马铃薯Y病毒衣壳蛋白抗原表位分析及其快速ELISA检测方法的建立

梁雨欣,吴建祥,李小宇,张春雨,侯吉超,周雪平,王永志

Mapping of Epitopes and Establishment of Rapid DAS-ELISA for Potato Virus Y Coat Protein

YuXin LIANG,JianXiang WU,XiaoYu LI,ChunYu ZHANG,JiChao HOU,XuePing ZHOU,YongZhi WANG

表2 3株MAb识别抗原表位分析

Table 2 Analysis of the antigen epitopes identified by 3 strains of PVY MAb

分段表达蛋白 Segmented expression protein 单克隆抗体Monoclonal antibody 3D3 9G6 3E4 PVY-CP1-180 + + + PVY-CP90-281 + + - PVY-CP1-59 - - + PVY-CP31-89 - - + PVY-CP90-148 + + - PVY-CP120-180 + - -