),解屹1,宋丽云1,申莉莉1,李莹1,王勇2,刘明宏3,刘东阳2,王小彦3,赵存孝4,王凤龙1(),杨金广1()
),Yi XIE1,LiYun SONG1,LiLi SHEN1,Ying LI1,Yong WANG2,MingHong LIU3,DongYang LIU2,XiaoYan WANG3,CunXiao ZHAO4,FengLong WANG1(),JinGuang YANG1()
图7. 重组载体表达及鉴定电泳图
Marker：2000 bp DNA marker;1：L4440-dsRdRP1461-1774重组载体的双酶切验证Identification of L4440-dsRdRP1461-1774 recombinant vector by double restriction endonuclease digestion;2：L4440-dsRdRP1461-1774菌液提取的核酸Nucleic acid extracted from L4440-dsRdRP1461-1774 bacterial solution;3：RNase-Free DNase处理过的L4440-dsRdRP1461-1774菌液核酸RNase-Free DNase treated L4440-dsRdRP1461-1774 bacterial solution nucleic acid;4：S1 Nuclease处理过的L4440-dsRdRP1461-1774菌液核酸S1 Nuclease treated L4440-dsRdRP1461-1774 bacterial solution nucleic acid;5：RNase-Free DNase、S1 Nuclease处理过的L4440- dsRdRP1461-1774菌液核酸RNase-Free DNase, S1 Nuclease treated L4440-dsRdRP1461-1774 bacterial solution nucleic acid;6：RNase-Free DNase、S1 Nuclease处理过的L4440菌液核酸样品RNase-Free DNase, S1 Nuclease-treated L4440 bacterial solution nucleic acid sample;7：菌液PCR检测 Fragments amplified by PCR from bacterial culture
Fig. 7. Expression and identification of recombinant vector
