小麦<i>Glu-3</i>位点基因拷贝数的变异分析

小麦Glu-3位点基因拷贝数的变异分析

陈璨,韩南南,刘洋,史晓维,司红起,马传喜

Analysis of Copy Number Variation of Glu-3 Locus in Common Wheat

Can CHEN,NanNan HAN,Yang LIU,XiaoWei SHI,HongQi SI,ChuanXi MA

表2 内参基因重复性检测Ct值变化情况

Table 2 Reproducibility of Ct measurements using RT-PCR for five levels of concentration of genomic DNA from common wheat

DNA浓度 DNA concentration (ng·μL^-1)	Ct值 Ct value			平均值 Average value	标准差 Standard deviation	方差 Variance	变异系数 Coefficient of variation (%)
DNA浓度 DNA concentration (ng·μL^-1)	1	2	3	平均值 Average value	标准差 Standard deviation	方差 Variance	变异系数 Coefficient of variation (%)
300	27.16	27.55	27.22	27.31	0.2100	0.0441	0.77
200	27.96	28.08	28.10	28.05	0.0757	0.0057	0.27
100	30.20	30.21	30.17	30.19	0.0208	0.0004	0.07
50	30.84	30.96	30.91	30.90	0.0603	0.0036	0.20
30	31.02	31.09	30.81	30.97	0.1457	0.0212	0.47