小麦Glu-3位点基因拷贝数的变异分析

陈璨,韩南南,刘洋,史晓维,司红起,马传喜

Analysis of Copy Number Variation of Glu-3 Locus in Common Wheat

Can CHEN,NanNan HAN,Yang LIU,XiaoWei SHI,HongQi SI,ChuanXi MA

表1 特异性引物和探针

Table 1 The specific primer and probe sequence

引物/探针名称 Primer/probe name 序列 Sequence (5′-3′) 退火温度 Annealing temperature (℃) Glu-A3F AGCAATCCCGCCATGAGTCA 60 Glu-A3R AGTGGTGGTTTCGTACAACG Glu-A3T AATGTGTCTCCCAACCCCAACAGCAG Glu-B3F CAAGTCATCTTTAGCAAGCATCAGG 59 Glu-B3R AAGGTCTTCATGGTGGACTAGTGTT Glu-B3T ATAGTAGCCAGGGCACCACCTCTTT Glu-D3F ACTAATCGAGCATATCCTAA 51 Glu-D3R GTTACATTGGGTTAGGTTT Glu-D3T AGCCTATACAAGGTTCCAAACTCGG Acc1F GCAATGTAGCTGCGCTTCAC Acc1R CCTGCAACCGTGGATTAAGT T-Acc1 ACAGTAGCAGCACCAACATAACCCACAGC