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桃小食心虫化学感受蛋白CSP16配体结合特性
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刘孝贺 1(  ),仇贵生 1,佟兆国 2,张怀江 1,闫文涛 1,岳强 1,孙丽娜 1( )
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Ligands Binding Characteristics of Chemosensory Protein CsasCSP16 of Carposina sasakii
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XiaoHe LIU 1( ),GuiSheng QIU 1,ZhaoGuo TONG 2,HuaiJiang ZHANG 1,WenTao YAN 1,Qiang YUE 1,LiNa SUN 1( )
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图1. CsasCSP16重组蛋白的原核表达和蛋白纯化
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Fig. 1. The prokaryotic expression and protein purification of recombinant protein CsasCSP16
A：CsasCSP16 cDNA核苷酸序列及其推导的氨基酸序列,信号肽序列用下划线标注,保守的半胱氨酸残基用圆圈标注,终止密码子用星号表示 Nucleotide and deduced amino acid sequence of CsasCSP14 cDNA. Signal peptide is underlined, conserved cysteine residues are marked in circles, the stop codons are denoted by asterisks [25]。B：CsasCSP16在大肠杆菌中的表达Induciton of the CsasCSP16 in E. coli;1：蛋白分子量标签 Protein molecular weight marker;4：未诱导对照组 Control group without induction;2、3和5：IPTG诱导的表达产物 Expressed products induced by IPTG。C：超声破菌结果The result of sonication;1：蛋白分子量标签 Protein molecular weight marker;2：重组菌的上清蛋白The soluble fraction of recombinant E. coli;3：包涵体 Inclusion body。D：CsasCSP16重组蛋白的纯化 Purification of the CsasCSP16 recombinant protein;1：蛋白分子量标签 Protein molecular weight marker;2：上柱前蛋白 The proteins before purified by NTA-Ni affinity chromatography;3：流出液 Outflow;4：30 mmol·L -1 咪唑 Imidazole at 30 mmol·L -1;5：300 mmol·L-1咪唑 Imidazole at 300 mmol·L-1
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