桃小食心虫化学感受蛋白CSP16配体结合特性
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刘孝贺,仇贵生,佟兆国,张怀江,闫文涛,岳强,孙丽娜
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Ligands Binding Characteristics of Chemosensory Protein CsasCSP16 of Carposina sasakii
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XiaoHe LIU,GuiSheng QIU,ZhaoGuo TONG,HuaiJiang ZHANG,WenTao YAN,Qiang YUE,LiNa SUN
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表1 重组蛋白CsasCSP16 与不同寄主挥发物和性信息素分子结合的数据
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Table 1 Binding data of recombinant CsasCSP16 with different host volatiles and sex pheromone molecules
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寄主挥发物和性信息素分子
Host volatile and sex pheromone molecule | CSP16 | 结合能
Binding energy (kJ·mol-1) | | IC50 (μmol·L-1) | Ki (μmol·L-1) | | 醇类 Alcohols | 2-己烯醇 2-hexenol | >50 | >50 | - | | 苯乙醇 Phenethyl alcohol | >50 | >50 | - | | 醛类 Aldehydes | 反-2-己烯醛 (E)-2-hexenal | >50 | >50 | - | | 己醛 Hexanal | >50 | >50 | - | | 苯甲醛 Benzaldehyde | >50 | >50 | - | | 庚醛 Heptanal | >50 | >50 | - | | 辛醛 Octanal | >50 | >50 | - | | 壬醛 Nonanal | >50 | >50 | - | | 癸醛 Decanal | >50 | >50 | - | | 酯类 Esters | 丁酸乙酯 Ethyl butyrate | >50 | >50 | - | | 乙酸丁酯 Butyl acetate | >50 | >50 | - | | 乙酸异戊酯 Isoamyl acetate | >50 | >50 | - | | 乙酸-顺-3-己烯酯 (Z)-3-hexenyl acetate | >50 | >50 | - | | 丁酸丁酯 Butyl butyrate | >50 | >50 | - | | 己酸乙酯 Ethyl hexanoate | >50 | >50 | - | | 乙酸己酯 Hexyl acetate | >50 | >50 | - | | 水杨酸甲酯 Methyl salicylate | 18.69 | 6.59 | -25.52 | | 庚酸乙酯 Ethyl heptanoate | >50 | >50 | - | | 己酸丁酯 Butyl hexanoate | >50 | >50 | - | | 茉莉酸甲酯 Methyl jasmonate | >50 | >50 | - | | 乙酸二甲基丁酯 Dimethyl butyl acetate | >50 | >50 | - | | 辛酸正丁酯 Butyl octanoate | >50 | >50 | - | | 庚酸丁酯 Butyl heptanoate | 19.74 | 6.73 | -21.76 | | 辛酸丙酯 Propyl octanoate | >50 | >50 | - | | 萜类 Terpenoids | α-蒎烯 α-pinene | 11.50 | 4.47 | -27.61 | | α-罗勒烯 α-ocimene | >50 | >50 | - | | 腈类 Benzonitriles | 苯甲腈 Benzonitrile | >50 | >50 | - | | 烷烃类 Alkanes | 癸烷 Decane | >50 | >50 | - | | 十四烷 Tetradecane | >50 | >50 | - | | 十五烷 Pentadecane | 7.90 | 3.50 | -24.27 | | 十六烷 Hexadecane | >50 | >50 | - | | 十八烷 Octadecane | >50 | >50 | - | | 酮类物质 Ketone | 6-甲基-5-庚烯-2-酮 6-methyl-5-hepten-2-one | 17.83 | 6.25 | -22.16 | | Z-7-二十烯-11-酮 Z-7-eicosen-11-one | >50 | >50 | - | | Z-7-十九烯-11-酮 Z-7-nonadecen-11-one | >50 | >50 | - |
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