图2. OsRRK1过量表达（OE-OsRRK1）转基因水稻植株的分子鉴定
A：OE-OsRRK1转基因植株中目的基因的PCR检测;M：MarkerIII;+：阳性质粒对照;-：未转基因水稻阴性对照;其余为部分OE-OsRRK1转基因水稻植株。B：OE-OsRRK1转基因植株的Southern blot分析。C：OE-OsRRK1转基因植株的实时荧光定量PCR分析,**在0.01水平的差异显著性。下同。D：OE-OsRRK1转基因植株的Northern blot分析。WT：野生型合江19;OE-1—OE-25：部分OE-OsRRK1转基因水稻植株,其中OE-5、OE-7、OE-16、OE-19、OE-21、OE-22、OE-24和OE-25为单拷贝株系,OE-5：单拷贝表达量程度低的株系;OE-22：单拷贝表达量程度中等的株系;OE-25：单拷贝表达量程度高的株系

Fig. 2. Molecular identification of transgenic rice plants with OsRRK1 overexpression (OE-OsRRK1)
A: PCR detection of target genes in OE-OsRRK1 transgenic plants; M: MarkerIII; +: Positive plasmid control; -: Non-transgenic rice negative control; The rest are some OE-OsRRK1 transgenic rice plants. B: Southern blot analysis of OE-OsRRK1 transgenic plants. C: Fluorescence quantitative PCR analysis of OE-OsRRK1 transgenic plants; ** t-test, P<0.01. The same as below. D: Northern blot analysis of OE-OsRRK1 transgenic plants; WT: Wild-type Hejiang 19; OE-1-OE-25: Part of OE-OsRRK1 transgenic rice plants, in which OE-5, OE-7, OE-16, OE-19, OE-21, OE-22, OE-24, OE-25 were single copy strains; OE-5: Strains with single copy of low expression level; OE-22: Single copy of medium expression level of strains; OE-25: Strains with single copy of high expression strains