利用第三代纳米孔长读段测序技术构建和注释蜜蜂球囊菌的全长转录组

杜宇,祝智威,王杰,王秀娜,蒋海宾,范元婵,范小雪,陈华枝,隆琦,蔡宗兵,熊翠玲,郑燕珍,付中民,陈大福,郭睿

Construction and Annotation of Ascosphaera apis Full-Length Transcriptome Utilizing Nanopore Third-Generation Long-Read Sequencing Technology

DU Yu,ZHU ZhiWei,WANG Jie,WANG XiuNa,JIANG HaiBin,FAN YuanChan,FAN XiaoXue,CHEN HuaZhi,LONG Qi,CAI ZongBing,XIONG CuiLing,ZHENG YanZhen,FU ZhongMin,CHEN DaFu,GUO Rui

表2 全长有效读段的信息概览

Table 2 Overview of full-length clean reads

样品 Sample 有效读段数 Number of clean reads 全长有效读段数 Number of full-length clean reads 全长有效读段数的占比 Percentage of full-length clean reads (%) 球囊菌菌丝Aam 5 669 436 4 497 102 79.32 球囊菌孢子Aas 6 233 159 4 963 101 79.62