利用第三代纳米孔长读段测序技术构建和注释蜜蜂球囊菌的全长转录组

杜宇,祝智威,王杰,王秀娜,蒋海宾,范元婵,范小雪,陈华枝,隆琦,蔡宗兵,熊翠玲,郑燕珍,付中民,陈大福,郭睿

Construction and Annotation of Ascosphaera apis Full-Length Transcriptome Utilizing Nanopore Third-Generation Long-Read Sequencing Technology

DU Yu,ZHU ZhiWei,WANG Jie,WANG XiuNa,JIANG HaiBin,FAN YuanChan,FAN XiaoXue,CHEN HuaZhi,LONG Qi,CAI ZongBing,XIONG CuiLing,ZHENG YanZhen,FU ZhongMin,CHEN DaFu,GUO Rui

表1 纳米孔长读段测序产生的原始读段信息概要

Table 1 Summary of raw reads produced from nanopore long-read sequencing

样品 Sample 原始读段数 Number of raw reads 碱基数 Number of bases 居中长度 N50 (bp) 平均长度 Mean length (bp) 最大长度 Maximum length (bp) 平均质量值 Mean Q score 球囊菌菌丝Aam 6 321 704 6 271 320 854 1 094 992 9 421 Q10 球囊菌孢子Aas 6 259 727 6 553 996 867 1 157 1 047 13 060 Q10