TETs与细胞程序性死亡相关基因在山羊妊娠早期输卵管及子宫角的表达

赵乐,杨海丽,李佳璐,杨永恒,张蓉,程文强,成磊,赵永聚

Expression Patterns of TETs and Programmed Cell Death Related Genes in Oviduct and Uterus of Early Pregnancy Goats

ZHAO Le,YANG HaiLi,LI JiaLu,YANG YongHeng,ZHANG Rong,CHENG WenQiang,CHENG Lei,ZHAO YongJu

表1 qRT-PCR引物序列

Table 1 The information of primers used in qRT-PCR

基因名称 Gene name 引物序列（5'→3'） Primer sequence（5'→3'） 产物长度 Product size (bp) β-actin F：TGATATTGCTGCGCTCGTGGT R：GTCAGGATGCCTCTCTTGCTC 189 WNT5a F：GCAAGGGCAATGTCTTCCA R：TTCATACCTAGCGACCACCAA 104 OPN F: GTTAAACCGACCAGTTCTGGCA R: GTTGTCATCAGTTTCCTCAGAGG 148 NF-kB F: GTGTAAAGAAGCGGGACTTGG R: GCGGTTGTCAAAGATGGGATG 203 Caspase9 F: CAATAGCAACCCTGAGACGGA R: AAAGCCTGGGAAGGTGGAGTA 130 GSDMD F: TTATTGGCTCTGACTGGGACG R: GGCAAAGCTGAAGCACGAAC 128 BCL2 F: GAAACCCCGCCACGAATTA R: CCAGCCAACAGTACGGAACAA 96 BAX F: TCTCCCCGAGAGGTCTTTTT R: TGATGGTCCTGATCAACTCG 151 NLRP3 F: CCGTCTGGGTGAGAGCGTGAA R: TCCTGTTGGCTCCTGTGTTCCT 78 Caspase1 F: GGATACAATAAATGGCTTGCTGG R: CTCGGGCTTTATCCATAGTTGT 110 TNF-α F: CCACGTTGTAGCCAACATCAG R: AGATGAGGTAAAGCCCGTCAG 134 VEGFA F: GTGACCCAGCACAGTTCCTCTT R: TTCCGGGCTCGGTGATTTAG 74 TET1 F: CATCACTGTCCGTCTTTGGA R: ACTTTCCTGTGCCTTTGTGG 126 TET2 F: GATACCATCTCCGTCTCCCATT R: CTTGCTGTCTCCATTCACTTCC 109 TET3 F: CCTTCTCCTTCGGTTGTTCCTG R: CACTTCTTCCTCTTTGGGGTTGTC 113