绿僵菌黏附素MAD1体外表达及诱导花生响应的作用

闫多子,蔡霓,王峰,农向群,王广君,涂雄兵,张泽华

Expression in vitro of Metarhizium anisopliae Adhesin MAD1 and Its Effect on Inducing Response in Peanut

YAN DuoZi,CAI Ni,WANG Feng,NONG XiangQun,WANG GuangJun,TU XiongBing,ZHANG ZeHua

表1 候选基因及其特异性引物

Table 1 Candidate genes and specific primers

基因Gene	引物序列Primer sequence	基因Gene	引物序列Primer sequence
CERK1	5′-CTACTCAGCCATTGCCAGTG	RPK	5′-AAGGTGGAGTTTGTTATGCC
CERK1	5′-CAGTCCTAAGAGGGTATGTGATGA	RPK	5′-CCTAAGGACTTGAGACCCTG
PTi1	5′-TTTGACCTGGGCACAGAGAG	RML1A	5′-CAGTGAGCGTTGCGAGATAC
PTi1	5′-GACACGGGTGGAATGAAGAC	RML1A	5′-TTGGGTTTCTTTCAGCGACT
SCW1	5′-GAGTAAGCGATTGCGGACAG	CDPK	5′-CAAGACCATTGTTGAGGTTG
SCW1	5′-TGTTCTCTCCCAAGTTCCCA	CDPK	5′-CTCCACACATCTGATTCTGG
MMK1	5′-AACTCGGAGACGAATGAGCA	MAPK	5′-CTTCCACAATGTCCAAAGCA
MMK1	5′-TTGGTGAAGGTCAGTGTCCA	MAPK	5′-CCAGATGAGTTCCTTGATGTC
MYB86	5′-CCACAACAGACAACAGTTTACG	RPL7	5′-AGAAGAGGGAGGAGGAATGG
MYB86	5′-TAGAGCACCATCTCCTACCTC	RPL7	5′-GGATGCGGATGATAAACAGG